Information on EC 1.14.13.34 - leukotriene-E4 20-monooxygenase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
1.14.13.34
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RECOMMENDED NAME
GeneOntology No.
leukotriene-E4 20-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14-tetraenoate + NADPH + H+ + O2 = 20-hydroxyleukotriene E4 + NADP+ + H2O
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arachidonic acid metabolism
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SYSTEMATIC NAME
IUBMB Comments
(7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14-tetraenoate,NADPH:oxygen oxidoreductase (20-hydroxylating)
Also acts on N-acetyl-leukotriene E4, but more slowly. Not identical with EC 1.14.13.30 leukotriene-B4 20-monooxygenase.
CAS REGISTRY NUMBER
COMMENTARY hide
111940-51-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14-tetraenoate + NADPH + O2
20-hydroxy-leukotriene E4 + NADP+ + H2O
show the reaction diagram
N-acetyl-leukotriene E4 + NADPH + O2
N-acetyl-omega-hydroxy-leukotriene E4 + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(7E,9E,11Z,14Z)-(5S,6R)-6-(cystein-S-yl)-5-hydroxyicosa-7,9,11,14-tetraenoate + NADPH + O2
20-hydroxy-leukotriene E4 + NADP+ + H2O
show the reaction diagram
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metabolism of leukotriene E4 and N-acetyl-leukotriene E4 in rat liver microsomes
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyeicosa-6,8,10,14-tetraenoate
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competitively inhibits leukotriene E4 omega-oxidation
Halothane
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induces a transient decrease in leukotriene omega-oxidation both in vivo and in vitro, halothane metabolism-dependent trifluoracetylation of hepatic target proteins might play a role in the inactivation of the enzyme
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyeicosa-6,8,10,14-tetraenoate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the specific activity is about 12times lower than for liver
Manually annotated by BRENDA team
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the specific activity is about 12times lower than for liver
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE