Information on EC 1.14.13.57 - dihydrochelirubine 12-monooxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.57
-
RECOMMENDED NAME
GeneOntology No.
dihydrochelirubine 12-monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dihydrochelirubine + NADPH + H+ + O2 = 12-hydroxydihydrochelirubine + NADP+ + H2O
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sanguinarine and macarpine biosynthesis
-
-
Isoquinoline alkaloid biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
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-
SYSTEMATIC NAME
IUBMB Comments
dihydrochelirubine,NADPH:oxygen oxidoreductase (12-hydroxylating)
A heme-thiolate protein (P-450)
CAS REGISTRY NUMBER
COMMENTARY hide
158736-41-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Thalictrum bulgaricum
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dihydrochelirubine + NADH + O2
12-hydroxydihydrochelirubine + NAD+
show the reaction diagram
Thalictrum bulgaricum
-
at 83% of the activity with NADPH
-
-
dihydrochelirubine + NADPH + O2
12-hydroxydihydrochelirubine + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dihydrochelirubine + NADPH + O2
12-hydroxydihydrochelirubine + NADP+
show the reaction diagram
Thalictrum bulgaricum
-
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
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-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
Thalictrum bulgaricum
-
dependent on
-
NADH
Thalictrum bulgaricum
-
dependent on NADPH or NADH
NADPH
Thalictrum bulgaricum
-
dependent on NADPH or NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Methyl-1,2-di(3-pyridyl)-1-propanone
Thalictrum bulgaricum
-
i.e. methyrapone
5-hydroxy-1,4-naphthoquinone
Thalictrum bulgaricum
-
i.e. juglone
CO
Thalictrum bulgaricum
-
inhibition in the dark, effect is partly reversible upon illumination
cytochrome c
Thalictrum bulgaricum
-
-
ketoconazole
Thalictrum bulgaricum
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
Thalictrum bulgaricum
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Thalictrum bulgaricum
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Thalictrum bulgaricum
-
yeast-elicited cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Thalictrum bulgaricum
-
associated with
-
Manually annotated by BRENDA team