Information on EC 1.14.13.64 - 4-hydroxybenzoate 1-hydroxylase

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The expected taxonomic range for this enzyme is: Candida parapsilosis

EC NUMBER
COMMENTARY hide
1.14.13.64
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RECOMMENDED NAME
GeneOntology No.
4-hydroxybenzoate 1-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-hydroxybenzoate + NAD(P)H + 2 H+ + O2 = hydroquinone + NAD(P)+ + H2O + CO2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzoate,NAD(P)H:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Requires FAD. The enzyme from Candida parapsilosis is specific for 4-hydroxybenzoate derivatives and prefers NADH to NADPH as electron donor.
CAS REGISTRY NUMBER
COMMENTARY hide
134214-78-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3,5,6-Tetrafluoro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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2,3,5,6-Tetrafluoro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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2,4-dihydroxybenzoate + NAD(P)H + H+ + O2
benzene-1,2,4-triol + NAD(P)+ + H2O + CO2
show the reaction diagram
2,4-Dihydroxybenzoate + NADH + O2
1,2,4-Trihydroxybenzene + NAD+ + CO2
show the reaction diagram
2,4-dihydroxybenzoate + NADPH + O2
1,2,4-trihydroxybenzene + NADP+ + CO2
show the reaction diagram
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2,5-Difluoro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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2,5-Difluoro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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2,6-Difluoro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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2,6-Difluoro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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2-chloro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
2-Chloro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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2-fluoro-4-hydroxybenzoate + NADH + O2
2-fluoro-1,4-dihydroxybenzene + NAD+ + CO2
show the reaction diagram
2-fluoro-4-hydroxybenzoate + NADPH + O2
2-fluoro-1,4-dihydroxybenzene + NADP+ + CO2
show the reaction diagram
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3,4-dihydroxybenzoate + NAD(P)H + H+ + O2
benzene-1,2,4-triol + NAD(P)+ + H2O + CO2
show the reaction diagram
3,4-Dihydroxybenzoate + NADH + O2
1,2,4-Trihydroxybenzene + NAD+ + CO2
show the reaction diagram
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3,4-Dihydroxybenzoate + NADPH + O2
1,2,4-Trihydroxybenzene + NADP+ + CO2
show the reaction diagram
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3,5-Difluoro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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3,5-Difluoro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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3-Amino-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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3-Amino-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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3-Chloro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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3-Chloro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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3-Fluoro-4-hydroxybenzoate + NADH + O2
?
show the reaction diagram
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3-Fluoro-4-hydroxybenzoate + NADPH + O2
?
show the reaction diagram
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4-Hydroxy-3-methoxybenzoate + NADH + O2
?
show the reaction diagram
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4-Hydroxy-3-methoxybenzoate + NADPH + O2
?
show the reaction diagram
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4-hydroxy-3-nitrobenzoate + NAD(P)H + O2
2-nitrobenzene-1,4-diol + NAD(P)+ + CO2
show the reaction diagram
4-Hydroxy-3-nitrobenzoate + NADH + O2
?
show the reaction diagram
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4-Hydroxy-3-nitrobenzoate + NADPH + O2
?
show the reaction diagram
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4-hydroxybenzoate + NAD(P)H + H+ + O2
hydroquinone + NAD(P)+ + H2O + CO2
show the reaction diagram
4-Hydroxybenzoate + NAD(P)H + O2
?
show the reaction diagram
4-Hydroxybenzoate + NADH + O2
1,4-Dihydroxybenzene + NAD+ + CO2
show the reaction diagram
4-hydroxybenzoate + NADH + O2
hydroquinone + NAD+ + CO2
show the reaction diagram
4-Hydroxybenzoate + NADPH + O2
1,4-Dihydroxybenzene + NADP+ + CO2
show the reaction diagram
4-hydroxybenzoate + NADPH + O2
hydroquinone + NADP+ + CO2
show the reaction diagram
protocatechuate + NAD(P)H + H+ + O2
?
show the reaction diagram
tetrafluoro-4-hydroxybenzoate + NAD(P)H + O2
2,3,5,6-tetrafluorohydroquinone + NAD(P)+ + CO2
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,4-dihydroxybenzoate + NAD(P)H + H+ + O2
benzene-1,2,4-triol + NAD(P)+ + H2O + CO2
show the reaction diagram
3,4-dihydroxybenzoate + NAD(P)H + H+ + O2
benzene-1,2,4-triol + NAD(P)+ + H2O + CO2
show the reaction diagram
4-hydroxybenzoate + NAD(P)H + H+ + O2
hydroquinone + NAD(P)+ + H2O + CO2
show the reaction diagram
4-Hydroxybenzoate + NAD(P)H + O2
?
show the reaction diagram
protocatechuate + NAD(P)H + H+ + O2
?
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3,5-Dichloro-4-hydroxybenzoate
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4-Hydroxy-3,5-dinitrobenzoate
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4-Hydroxyisophthalate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
2,3,5,6-Tetrafluoro-4-hydroxybenzoate
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0.007
2,4-Dihydroxybenzoate
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0.005
2,5-Difluoro-4-hydroxybenzoate
0.004
2,6-Difluoro-4-hydroxybenzoate
0.025
2-Chloro-4-hydroxybenzoate
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0.018
2-Fluoro-4-hydroxybenzoate
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0.009
3,4-dihydroxybenzoate
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0.013
3-amino-4-hydroxybenzoate
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4-hydroxy-3-nitrobenzoate
0.008
3-Fluoro-4-hydroxybenzoate
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0.125
4-hydroxy-3-methoxybenzoate
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0.01
4-hydroxybenzoate
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0.019
NADH
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0.169
NADPH
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.5
2,3,5,6-Tetrafluoro-4-hydroxybenzoate
Candida parapsilosis
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19.8
2,4-Dihydroxybenzoate
Candida parapsilosis
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12
2,5-Difluoro-4-hydroxybenzoate
Candida parapsilosis
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8.5
2,6-Difluoro-4-hydroxybenzoate
Candida parapsilosis
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9.6
2-Chloro-4-hydroxybenzoate
Candida parapsilosis
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14
2-Fluoro-4-hydroxybenzoate
Candida parapsilosis
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16.8
3,4-dihydroxybenzoate
Candida parapsilosis
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6.5
3,5-Difluoro-4-hydroxybenzoate
Candida parapsilosis
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8.3
3-amino-4-hydroxybenzoate
Candida parapsilosis
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17
3-Chloro-4-hydroxybenzoate
Candida parapsilosis
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18.1
3-Fluoro-4-hydroxybenzoate
Candida parapsilosis
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11.8
4-hydroxy-3-methoxybenzoate
Candida parapsilosis
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19.8
4-Hydroxy-3-nitrobenzoate
Candida parapsilosis
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10
4-hydroxybenzoate
Candida parapsilosis
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE