Information on EC 1.14.13.73 - tabersonine 16-hydroxylase

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The expected taxonomic range for this enzyme is: Catharanthus roseus

EC NUMBER
COMMENTARY hide
1.14.13.73
-
RECOMMENDED NAME
GeneOntology No.
tabersonine 16-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
tabersonine + NADPH + H+ + O2 = 16-hydroxytabersonine + NADP+ + H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
vindoline and vinblastine biosynthesis
-
-
Indole alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
tabersonine,NADPH:oxygen oxidoreductase (16-hydroxylating)
A heme-thiolate protein (P-450).
CAS REGISTRY NUMBER
COMMENTARY hide
250378-34-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydro-3-hydroxytabersonine + NADPH + H+ + O2
?
show the reaction diagram
low activity
-
-
?
2,3-dihydrotabersonine + NADPH + H+ + O2
?
show the reaction diagram
low activity
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-dihydro-3-hydroxytabersonine + NADPH + H+ + O2
?
show the reaction diagram
C0KYN4
low activity
-
-
?
2,3-dihydrotabersonine + NADPH + H+ + O2
?
show the reaction diagram
C0KYN4
low activity
-
-
?
tabersonine + NADPH + H+ + O2
16-hydroxytabersonine + NADP+ + H2O
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
clotrimazole
-
50% inhibition at 0.05 mM
CO
-
concentration dependent competitive inhibition, 77% inhibition at 90%
cytochrome c
-
50% inhibition at 0.001 mM
miconazole
-
50% inhibition at 0.3 mM
tabersonine
-
inhibition above 0.03 mM
tricliphane
-
50% inhibition at 0.5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
NADPH
-
-
0.00007 - 0.011
tabersonine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
predominantly
Manually annotated by BRENDA team
isoform T16H1, CYP71D351
Manually annotated by BRENDA team
highest expression of isoform T16H2, CYP71D12 in young leaves
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix
Manually annotated by BRENDA team
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
expressed in Saccharomyces cerevisiae WAT11 cells
T16H expression analysis in leaves, overview, recombinant expression in Escherichia coli
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