Information on EC 1.14.19.12 - acyl-lipid omega-(9-4) desaturase

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The expected taxonomic range for this enzyme is: Chlamydomonas reinhardtii

EC NUMBER
COMMENTARY hide
1.14.19.12
-
RECOMMENDED NAME
GeneOntology No.
acyl-lipid omega-(9-4) desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = coniferonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
(2)
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-
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linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = pinolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
(1)
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pinolenate and coniferonate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
acyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase [omega(9-4),omega(9-5) cis-dehydrogenating]
The enzyme, characterized from the green alga Chlamydomonas reinhardtii, is a front-end desaturase that introduces a cis double bond in omega9 unsaturated C18 or C20 fatty acids incorporated into lipids, at a position 4 carbon atoms from the existing omega9 bond, towards the carboxy end of the fatty acid (at the omega13 position). When acting on 20:2Delta(11,14) and 20:3Delta(11,14,17) substrates it introduces the new double bond between carbons 7 and 8. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor for the active site of the desaturase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
show the reaction diagram
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
show the reaction diagram
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
show the reaction diagram
low activity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
show the reaction diagram
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
show the reaction diagram
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
show the reaction diagram
Q2HWK7
low activity
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris