Information on EC 1.14.19.B5 - 7-chloro-L-tryptophan 6-halogenase

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The expected taxonomic range for this enzyme is: Kutzneria sp. 744

EC NUMBER
COMMENTARY hide
1.14.19.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
7-chloro-L-tryptophan 6-halogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+ = 6,7-dichloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
7-chloro-L-tryptophan:FADH2 oxidoreductase (6-halogenating)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+
6,7-dichloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
L-tryptophan + FADH2 + Cl- + O2 + H+
6-chloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
kcat/Km for the halogenation of L-tryptophan is less than 1% compared to the value for the halogenation 7-chloro-L-tryptophan
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-
?
additional information
?
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KtzR is not active for introduction of chloride to piperazic acid or gamma,delta-dehydropiperazic acid
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7-chloro-L-tryptophan + FADH2 + Cl- + O2 + H+
6,7-dichloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
A8CF74
the enzyme is involved in the biosynthesis of 6,7-dichloro-L-tryptophan, a component of kutznerides (antifungal nonribosomal hexadepsipeptides)
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.114
7-chloro-L-tryptophan
pH 7.0, 22C
0.808
L-tryptophan
pH 7.0, 22C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
7-chloro-L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22C
0.0013
L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
7-chloro-L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22C
11727
0.0016
L-tryptophan
Kutzneria sp. 744
A8CF74
pH 7.0, 22C
119
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE