Information on EC 1.14.19.B6 - tryptophan 6-halogenase

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The expected taxonomic range for this enzyme is: Streptomyces toxytricini

EC NUMBER
COMMENTARY hide
1.14.19.B6
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
tryptophan 6-halogenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-tryptophan + FADH2 + Cl- + O2 + H+ = 6-chloro-D-tryptophan + FAD + 2 H2O
show the reaction diagram
L-tryptophan + FADH2 + Cl- + O2 + H+ = 6-chloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
tryptophan:FADH2 oxidoreductase (6-halogenating)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-tryptophan + FADH2 + Cl- + O2 + H+
6-chloro-D-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
?
L-tryptophan + FADH2 + Br- + O2 + H+
6-bromo-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
?
L-tryptophan + FADH2 + Cl- + O2 + H+
6-chloro-L-tryptophan + FAD + 2 H2O
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
L-tryptophan
pH 6.0, 28C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0255
L-tryptophan
Streptomyces toxytricini
E9P162
pH 6.0, 28C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
L-tryptophan
Streptomyces toxytricini
E9P162
pH 6.0, 28C
119
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
pH 5.0: about 75% of maximal activity, pH 7.0: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
35C: about 75% of maximal activity, 45C: about 75% of maximal activity
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 59000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His6-tagged enzyme is expressed in Escherichia coli RIL