Information on EC 1.14.99.19 - plasmanylethanolamine desaturase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.99.19
-
RECOMMENDED NAME
GeneOntology No.
plasmanylethanolamine desaturase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + AH2 + O2 = O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + A + 2 H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
-
redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ether lipid metabolism
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-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine,hydrogen-donor:oxygen oxidoreductase
Requires NADPH or NADH. May involve cytochrome b5. Requires Mg2+ and ATP.
CAS REGISTRY NUMBER
COMMENTARY hide
39391-13-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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plasmanylethanolamine desaturase is a key enzyme for the biosynthesis of one of the most abundant phospholipidsin the body, the plasmalogens. Ether lipid metabolism including biosynthesis and degradation of platelet-activating factor and plasmalogens, overview
additional information
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the enzyme is a defined enzyme without assigned sequence, these kind of enzymes are known as orphan enzymes
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-hexadecyl-2-acyl glycero-3-phosphoethanolamine + NADH + O2
?
show the reaction diagram
-
in situ in the microsomes via acylation of 1-hexadecyl-glycero-3-phosphoethanolamine
-
-
?
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + AH2 + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + A + 2 H2O
show the reaction diagram
-
i.e. plasmanylethanolamine
i.e. plasmenylethanolamine
-
?
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
show the reaction diagram
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + reduced cytochrome b5 + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized cytochrome b5 + 2 H2O
show the reaction diagram
-
i.e. plasmanylethanolamine, the reaction involves reaction involving cytochrome b5 reductase, molecular oxygen and a reduction equivalent
i.e. plasmenylethanolamine
-
?
O-1-hexadecyl-rac-glycero-3-phosphoethanolamine + NADPH + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + AH2 + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + A + 2 H2O
show the reaction diagram
-
i.e. plasmanylethanolamine
i.e. plasmenylethanolamine
-
?
O-1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine + electron donor + O2
O-1-alk-1-enyl-2-acyl-sn-glycero-3-phosphoethanolamine + oxidized electron donor + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome b5
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NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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cholate
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weak inhibition
oleate
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weak inhibition
p-chloromercuribenzoate
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taurocholate
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
catalase
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heat-lable factor of high molecular weight
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contained in the 100000 * g supernatant
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Mg2+
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stimulates the conversion by a factor of 2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000028
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microsomes plus soluble fraction, enzyme requires a heat-labile factor of high molecular weight contained in the soluble fraction
0.0000057 - 0.0000064
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two-dimensional TLC method
0.0000066
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hydrolysis followed by solvent partition
0.000025
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tumor cells of rat on fat-free diet
0.000029
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tumor cells of rat on normal diet
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
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phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Fischer R-3259 sarcoma
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity loss at -23C, should be used freshly
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-23C, postmitochondrial supernatant stable for two weeks
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-23C, supernatant
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-70C, TES buffer, 0.25 M sucrose, 1 mM EDTA
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