Information on EC 1.14.99.2 - kynurenine 7,8-hydroxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.99.2
-
RECOMMENDED NAME
GeneOntology No.
kynurenine 7,8-hydroxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
kynurenate + AH2 + O2 = 7,8-dihydro-7,8-dihydroxykynurenate + A
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tryptophan metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
kynurenate,hydrogen-donor:oxygen oxidoreductase (hydroxylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
9029-63-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 11299b
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-
Manually annotated by BRENDA team
overview
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
show the reaction diagram
kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
show the reaction diagram
kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetate
-
inhibition reversed by addition of GSH or cysteine
Kynurenate
-
competitive inhibition at 0.2 mM
p-chloromercuribenzoate
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inhibition reversed by addition of GSH or cysteine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008
Kynurenate
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
stable in presence 10 mM cysteine
390584
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
5 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes activity
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cysteine stabilizes activity
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GSH stabilizes activity
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precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity
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thioglycolate stabilizes activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, ammonium sulfate fractions, loss of activity after a few days
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-10°C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE