Information on EC 1.17.99.4 - uracil/thymine dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.17.99.4
-
RECOMMENDED NAME
GeneOntology No.
uracil/thymine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thymine + H2O + acceptor = 5-methylbarbiturate + reduced acceptor
show the reaction diagram
-
-
-
-
uracil + H2O + acceptor = barbiturate + reduced acceptor
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
uracil degradation II (oxidative)
-
-
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
uracil:acceptor oxidoreductase
Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 3.5.2.1 (barbiturase) and EC 3.5.1.95 (N-malonylurea hydrolase). Mammals, plants and other microorganisms utilize the reductive pathway, comprising EC 1.3.1.1 [dihydrouracil dehydrogenase (NAD+)] or EC 1.3.1.2 [dihydropyrimidine dehydrogenase (NADP+)], EC 3.5.2.2 (dihydropyrimidinase) and EC 3.5.1.6 (beta-ureidopropionase), with the ultimate degradation products being an L-amino acid, NH3 and CO2 [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9029-00-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Aerobic soil bacterium
strain U-1
-
-
Manually annotated by BRENDA team
Aerobic soil bacterium U-1
strain U-1
-
-
Manually annotated by BRENDA team
strain 161
-
-
Manually annotated by BRENDA team
strain 161
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
JCM 3132
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-aminouracil + acceptor
?
show the reaction diagram
thymine + acceptor
5-methylbarbiturate + reduced acceptor
show the reaction diagram
thymine + H2O + acceptor
5-methylbarbiturate + reduced acceptor
show the reaction diagram
uracil + acceptor
barbiturate + reduced acceptor
show the reaction diagram
uracil + H2O + acceptor
barbiturate + reduced acceptor
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thymine + H2O + acceptor
5-methylbarbiturate + reduced acceptor
show the reaction diagram
uracil + acceptor
barbiturate + reduced acceptor
show the reaction diagram
-
reaction in two-step oxidative catabolism of uracil in prokaryotes
-
?
uracil + H2O + acceptor
barbiturate + reduced acceptor
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
-
required as electron acceptor, promotes barbiturate formation better than methylene blue
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methylene blue
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
thymine
-
-
0.131
Uracil
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10
-
about half-maximal activity at pH 8.0 and 10 with thymine as substrate
8 - 9.7
-
about half-maximal activity at pH 8.0 and 9.7 with uracil as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22 - 25
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
90% loss of activity
209264
9
-
most stable at -10°C, no appreciable loss of activity for several months
209264
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme in cell-free extract is unstable, activity totally diappears within 3 days
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, most stable at pH 9.0, no appreciable loss of activity for several months
-
-20°C, 80% loss of activity in crude extracts within two months
Aerobic soil bacterium
-