Information on EC 1.2.1.20 - glutarate-semialdehyde dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.20
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RECOMMENDED NAME
GeneOntology No.
glutarate-semialdehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-oxopentanoate + NAD+ + H2O = glutarate + NADH + 2 H+
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-lysine degradation III
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lysine metabolism
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Lysine degradation
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SYSTEMATIC NAME
IUBMB Comments
glutarate-semialdehyde:NAD+ oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-99-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
denitrifiying, strain pF6
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-hydroxybutyraldehyde + NAD+ + H2O
2-hydroxybutanoate + NADH
show the reaction diagram
-
low activity
-
-
?
2-oxoglutaric semialdehyde + NAD+ + H2O
2-oxoglutarate + NADH
show the reaction diagram
-
-
-
-
?
5-hydroxyvaleraldehyde + NAD+ + H2O
5-hydroxypentanoate + NADH + H+
show the reaction diagram
-
low activity
-
-
?
butyraldehyde + NAD+ + H2O
butanoate + NADH
show the reaction diagram
-
low activity
-
-
?
DL-glyceraldehyde + NAD+ + H2O
glycerate + NADH
show the reaction diagram
glutaraldehyde + NAD+ + H2O
glutarate semialdehyde + NADH
show the reaction diagram
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low activity
-
-
?
glutarate semialdehyde + NAD+ + H2O
glutarate + NADH
show the reaction diagram
glutarate semialdehyde + NADP+ + H2O
glutarate + NADPH
show the reaction diagram
glutarate semialdehyde + NADP+ + H2O
glutaric acid + NADPH + H+
show the reaction diagram
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-
-
-
?
heptaldehyde + NAD+ + H2O
heptanoate + NADH
show the reaction diagram
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low activity
-
-
?
valeraldehyde + NAD+ + H2O
valeric acid + NADH + H+
show the reaction diagram
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low activity
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glutarate semialdehyde + NAD+ + H2O
glutarate + NADH
show the reaction diagram
glutarate semialdehyde + NADP+ + H2O
glutaric acid + NADPH + H+
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-hydroxy-2-ketoglutaric semialdehyde
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PCMB
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0.33 mM, complete inhibition
Valeraldehyde
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
Glutarate semialdehyde
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-
0.0078
NAD+
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 8.7
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pH 7.2: about 50% of maximal activity, pH 8.7: about 40% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
170000
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sucrose density gradient sedimentation, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 53000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 20 mm Tris-HCl buffer, pH 8.0
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0°C, unstable to storage, inclusion of 10% glycerol greatly improves stability
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mutant strain lacking delta-aminovalerate transaminase
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to homogeneity as a C-terminal histidine-tagged fusion protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BW25113(DE3) cells
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into pET21a(+) vector, proteins with a C-terminal His6-tag expressed in Escherichia coli BL21(DE3)
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Show AA Sequence (148 entries)
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