Information on EC 1.2.1.25 - 2-oxoisovalerate dehydrogenase (acylating)

Word Map on EC 1.2.1.25
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.25
-
RECOMMENDED NAME
GeneOntology No.
2-oxoisovalerate dehydrogenase (acylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-methyl-2-oxobutanoate + CoA + NAD+ = 2-methylpropanoyl-CoA + CO2 + NADH
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
oxidative decarboxylation
-
-
-
-
redox reaction
-
-
-
-
reduction
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-valine degradation I
-
-
valine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3-methyl-2-oxobutanoate:NAD+ 2-oxidoreductase (CoA-methyl-propanoylating)
Also acts on (S)-3-methyl-2-oxopentanoate and 4-methyl-2-oxopentanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
37211-61-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-methyl-2-oxobutanoate + CoA + NAD+
2-methylpropanoyl-CoA + CO2 + NADH
show the reaction diagram
-
specific for the L-isomer
-
?
4-methyl-2-oxopentanoate + CoA + NAD+
3-methyl-butanoyl-CoA + CO2 + NADH
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CoA
-
required
NAD+
-
required
additional information
-
CoA and NAD+ can be replaced by potassium ferricyanide but the activity is much lower; enzyme probably identical with EC 1.2.4.4
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoisopentanoate
-
competitive
iodoacetate
-
-
L-2-Oxo-3-methylpentanoate
-
competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11
CoA
-
-
1.66
L-alpha-keto-beta-methylvalerate
-
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
pH 6.0: about 25% of maximal activity, pH 7.5: about 45% of maximal activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Lactococci produce acetic and propionic acid during logarithmic growth and starvation