Information on EC 1.2.1.49 - 2-oxoaldehyde dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.49
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RECOMMENDED NAME
GeneOntology No.
2-oxoaldehyde dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 2-oxoaldehyde + NADP+ + H2O = a 2-oxo carboxylate + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methylglyoxal degradation II
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Pyruvate metabolism
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SYSTEMATIC NAME
IUBMB Comments
2-oxoaldehyde:NADP+ 2-oxidoreductase
Not identical with EC 1.2.1.23 2-oxoaldehyde dehydrogenase (NAD+).
CAS REGISTRY NUMBER
COMMENTARY hide
83588-97-0
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97162-76-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconic acid + NADPH + H+
show the reaction diagram
2-oxoaldehydes + NADP+ + H2O
2-oxo acids + NADPH
show the reaction diagram
methylglyoxal + NADP+ + H2O
pyruvate + NADPH
show the reaction diagram
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-keto-3-deoxy-D-glucose + NADP+ + H2O
2-keto-3-deoxy-D-gluconic acid + NADPH + H+
show the reaction diagram
methylglyoxal + NADP+ + H2O
pyruvate + NADPH
show the reaction diagram
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contributes to the detoxification of methylglyoxal
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?
methylglyoxal + NADP+ + H2O
pyruvate + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
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3-acetyl-pyridine adenine dinucleotide and thionicotinamide adenine dinucleotide are also effective oxidants
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Barbiturates
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dihydroxyacetone
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Glycine buffer
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complete blockage of the reaction
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hydrazine
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1-3 mM
N-ethylmaleimide
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1-3 mM
N-tris(hydroxymethyl)methylglycine
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Nitro-alcohols
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p-hydroxymercuribenzoate
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1-3 mM
Sodium bisulfite
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1 mM
Sodium cyanide
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1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-amino-2-methylpropan-1-ol
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activation of aldehyde oxidation
2-amino-2-methylpropane-1,3-diol
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activation of aldehyde oxidation
2-Aminobutan-1-ol
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activation of aldehyde oxidation
2-aminopropane
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activation of aldehyde oxidation
2-aminopropane-1,3-diol
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activation of aldehyde oxidation
Amines
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several amines stimulate activity
D,L-2-amino-1-propanol
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activation of aldehyde oxidation
DL-2-amino-1-propanol
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required, best activator of the reaction
L-2-Aminopropan-1-ol
L-serine methyl ester
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activation of aldehyde oxidation
Tris
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activation of aldehyde oxidation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
2-keto-3-deoxyglucose
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83
L-2-Aminopropan-1-ol
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amine as activator in aldehyde oxidation
8
L-serine methyl ester
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amine as activator in aldehyde oxidation
0.14 - 0.83
methylglyoxal
0.0045 - 0.8
NADP+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.23
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0.24
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additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.3
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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not active below
7.8
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not active below
8.5 - 10
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very low activity at physiological pH
9.3
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activity falls abruptly above
9.4
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activity increases up to, Tris buffer
10.5
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activity increases up to, carbonate buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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SDS-PAGE
42000
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gel filtration
50000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.3
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not stable above
288259
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol
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4°C, pH 7.4, 50 mM NaCl, 5 mM 2-amino-2-methylpropane-1,3-diol, 20 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200
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using ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50 column, Blue-dextran affinity column and CM-Sephadex C-50 column
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using ammonium sulfate fractionation and chromatography on DEAE-Sephadex column, Blue-dextran affinity column and CM-Sephadex column
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using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-200, hydroxylapatite, and DEAE-cellulose
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using treatment with acetone, ammonium sulfate fractionation, and gel filtration on Sephadex G-25
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
detection of three Mgd loci, which show a restrictive tissue expression, Mgd1 and Mgd2 are preferentially expressed in liver and kidney, Mgd3 is expressed apparently exclusively in heart
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