Information on EC 1.2.1.60 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.2.1.60
-
RECOMMENDED NAME
GeneOntology No.
5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ = 5-carboxymethyl-2-hydroxymuconate + NADH + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-hydroxyphenylacetate degradation
Tyrosine metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
5-carboxymethyl-2-hydroxymuconic-semialdehyde:NAD+ oxidoreductase
Involved in the tyrosine degradation pathway in Arthrobacter sp.
CAS REGISTRY NUMBER
COMMENTARY hide
63241-20-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PRL W15
-
-
Manually annotated by BRENDA team
strain PRL W15
-
-
Manually annotated by BRENDA team
strain C
-
-
Manually annotated by BRENDA team
strain M5a1
-
-
Manually annotated by BRENDA team
strain M5a1
-
-
Manually annotated by BRENDA team
strain NCIB 9865
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
show the reaction diagram
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+
5-carboxymethyl-2-hydroxymuconate + NADPH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
5-carboxymethyl-2-hydroxymuconate + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
competitive inhibition, Ki: 0.088 mM
m-hydroxybenzaldehyde
-
-
p-Hydroxybenzaldehyde
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.009
5-carboxymethyl-2-hydroxymuconate semialdehyde
0.012 - 0.052
NAD+
1.2 - 3.6
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40
5-carboxymethyl-2-hydroxymuconate semialdehyde
Pseudomonas putida
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102000
-
gel filtration
125000
-
gel filtration
200000
-
gel filtration
210000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable for 30 min
37
-
stable for 30 min
42
-
loss of activity after 10 min
47
-
complete loss of activity after 20 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
frozen, 12 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity, chromatography techniques
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