Information on EC 1.21.3.4 - sulochrin oxidase [(+)-bisdechlorogeodin-forming]

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The expected taxonomic range for this enzyme is: Penicillium glabrum

EC NUMBER
COMMENTARY hide
1.21.3.4
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RECOMMENDED NAME
GeneOntology No.
sulochrin oxidase [(+)-bisdechlorogeodin-forming]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 sulochrin + O2 = 2 (+)-bisdechlorogeodin + 2 H2O
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
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-
-
-
reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
asterrate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
sulochrin:oxygen oxidoreductase (cyclizing, (+)-specific)
Also acts on several diphenols and phenylenediamines, but has low affinity for these substrates. Involved in the biosynthesis of mould metabolites related to the antibiotic griseofulvin.
CAS REGISTRY NUMBER
COMMENTARY hide
82469-87-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CMI 96659
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-
Manually annotated by BRENDA team
strain CMI 96659
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-ascorbic acid + O2
?
show the reaction diagram
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-
-
-
?
catechol + O2
?
show the reaction diagram
dihydrogeodin + O2
(+)-geodin + H2O
show the reaction diagram
griseophenone A + O2
dehydrodemethylgriseofulvin + H2O
show the reaction diagram
hydroquinone + O2
?
show the reaction diagram
N,N,N',N'-tetramethyl-p-phenylenediamine + O2
?
show the reaction diagram
N,N-dimethyl-p-phenylenediamine + O2
?
show the reaction diagram
o-phenylenediamine + O2
?
show the reaction diagram
-
-
-
-
?
p-phenylenediamine + O2
?
show the reaction diagram
sulochrin + O2
(+)-bisdechlorogeodin + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulochrin + O2
(+)-bisdechlorogeodin + H2O
show the reaction diagram
-
main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sodium diethyldithiocarbamate
additional information
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not inhibited by 2 mM neocuproin, 5 mM, potassium cyanide or 10 mM EDTA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
150
(+)-ascorbic acid
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-
40
catechol
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-
60
hydroquinone
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-
1
N,N,N',N'-tetramethyl-p-phenylenediamine
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-
0.4
N,N-Dimethyl-p-phenylenediamine
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5
o-phenylenediamine
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2
p-phenylenediamine
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-
0.0065
sulochrin
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
157000
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gel filtration
230000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
10% glycerol stabilizes condiderably
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 5.8, citrate-phosphate buffer, several months, no loss in activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, hydroxyapatite, Phenyl-Sepharose, Mono p, Mono Q, TSK G 3000
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ammonium sulfate, pH 3.3, isoelectric focusing, gel filtration
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