Information on EC 1.23.1.4 - (-)-lariciresinol reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.23.1.4
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RECOMMENDED NAME
GeneOntology No.
(-)-lariciresinol reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-secoisolariciresinol + NADP+ = (-)-lariciresinol + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
justicidin B biosynthesis
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matairesinol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(+)-secoisolariciresinol:NADPH+ oxidoreductase
The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that also reduces (-)-pinoresinol [EC 1.23.1.3, (-)-pinoresinol reductase]. Isolated from the plants Thuja plicata (western red cedar) [1] and Linum corymbulosum [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the biosynthesis of justicidin B
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-lariciresinol + NADPH + H+
(+)-secoisolariciresinol + NADP+
show the reaction diagram
additional information
?
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the protein shows preference for (+)-pinoresinol (R,R configuration at C-atoms 8,8’) in the first reaction step, but preference for (-)-lariciresinol (S,S configuration at C-atoms 8,8’) in the second reaction step
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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not expressed in leaf and stem
Manually annotated by BRENDA team
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method, using 20% (w/v) polyethylene glycol 8000, 0.1 M MES, pH 6.2, 0.1 M NaCl, and 0.1 M calcium acetate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
metal chelate chromatography
Ni-NTA column chromatography
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POROS 20 HQ column chromatography and ADP-agarose column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in BL21 (DE3)-RIL cells
expressed in Escherichia coli B834-DE3 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
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expressed in Escherichia coli Nova Blue cells
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expressed in Escherichia coli strain HB101
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the plasmid is transferred into the disarmed Agrobacter tumefaciens strain GV3101 by triparental mating with Escherichia coli strain HB101 and then introduced into Linum usitatissimum (cv. Barbara) transgenic plants
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression of the PLR1 gene in the seed coat is down-regulated about 3fold when 0.01 mM fluridone is applied
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the expression of the PLR1 gene in the seed coat is up-regulated about 3fold by 0.1 mM exogenous abscisic acid. There is a high mid-maturation expression of PLR1 gene between developmental stage 2 and stage
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