Information on EC 1.3.1.15 - dihydroorotate dehydrogenase (NADP+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.15
-
RECOMMENDED NAME
GeneOntology No.
dihydroorotate dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-dihydroorotate + NADP+ = orotate + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-dihydroorotate:NADP+ oxidoreductase
Binds FMN and FAD [2]. Other class 1 dihydroorotate dehydrogenases use either fumarate (EC 1.3.98.1) or NAD+ (EC 1.3.1.14) as electron acceptor. The membrane bound class 2 dihydroorotate dehydrogenase (EC 1.3.5.2) uses quinone as electron acceptor .
CAS REGISTRY NUMBER
COMMENTARY hide
37255-27-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gram-negative, non-spore forming obligate aerobe
-
-
Manually annotated by BRENDA team
pseudomonad isolated from lake bank mud, capable of growing on salt medium with glucose, aspartate, glycerol or orotate as carbon source
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-fluoroorotate + NADPH
(S)-5-fluorodihydroorotate + NADP+
show the reaction diagram
-
enzyme exhibits very weak oxidase activity
-
?
orotate + NADPH
(S)-dihydroorotate + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
orotate + NADPH
(S)-dihydroorotate + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
ratio FAD/FMN 1:1 (probably)
FMN
-
ratio FAD/FMN 1:1 (probably)
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
Mg2+ has no effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-Dihydroxy-6-methylpyridine
-
7.8 mM, 22% inhibition
5-Methylorotic acid
-
6 mM, 63% inhibition
Barbituric acid
-
2 mM, 60% inhibition
p-chloromercuribenzoate
-
0.01 mM, 90% inhibition, 0.001 mM, 30% inhibition
Uracil-5-carboxylic acid
-
7.8 mM, 30% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
5-Fluoroorotate
-
-
0.2
NADPH
-
-
0.17
Orotate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.33 - 11.7
Orotate
Bacteria
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.19
-
enzyme activity in cells grown on orotate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
sodium phosphate buffer
7.7
-
Tris buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 8.8
-
at pH 6.7 and 8.8: about 25% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
8 min, 81% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 4 months, complete loss of activity
-
frozen or at 4°C
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE