Information on EC 1.3.1.46 - biochanin-A reductase

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The expected taxonomic range for this enzyme is: Fusarium striatum

EC NUMBER
COMMENTARY hide
1.3.1.46
-
RECOMMENDED NAME
GeneOntology No.
biochanin-A reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dihydrobiochanin A + NADP+ = biochanin A + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoflavonoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
dihydrobiochanin-A:NADP+ DELTA2-oxidoreductase
Some other isoflavones are reduced to the corresponding isoflavanones.
CAS REGISTRY NUMBER
COMMENTARY hide
112198-90-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,7,3'-trihydroxy-4'-methoxyisoflavone + NADPH + H+
2-dihydropratensein + NADP+
show the reaction diagram
-
trivial name pratensein, 78% of activity with biochanin A
-
?
5,7,3'-trihydroxyisoflavone + NADPH + H+
2-dihydrogenistein + NADP+
show the reaction diagram
-
trivial name genistein, 44% of activity with biochanin A
-
?
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6,7-dihydroxy-4'-methoxyisoflavone + NADPH + H+
(3S)-2-dihydrobiochanin A + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.043
Biochanin A
-
-
0.19
NADPH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
87000 - 88000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 43000-44000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
inactivation above
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-25°C, 20% v/v glycerol, with NADPH, but unstable with NADP+
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE