Information on EC 1.3.1.5 - cucurbitacin DELTA23-reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Cucurbita maxima

EC NUMBER
COMMENTARY hide
1.3.1.5
-
RECOMMENDED NAME
GeneOntology No.
cucurbitacin DELTA23-reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
redox reaction
-
-
reduction
-
-
SYSTEMATIC NAME
IUBMB Comments
23,24-dihydrocucurbitacin:NAD(P)+ DELTA23-oxidoreductase
Requires Mn2+. Fe2+ or Zn2+ can replace Mn2+ to some extent.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. Green Hubbard
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cucurbitacin A + NAD(P)H
23,24-dihydrocucurbitacin A + NAD(P)+
show the reaction diagram
-
very low activity
-
-
?
cucurbitacin B + NAD(P)H + H+
23,24-dihydrocucurbitacin B + NAD(P)+
show the reaction diagram
cucurbitacin C + NAD(P)H
23,24-dihydrocucurbitacin C + NAD(P)+
show the reaction diagram
-
very low activity
-
-
?
cucurbitacin D + NAD(P)H
23,24-dihydrocucurbitacin D + NAD(P)+
show the reaction diagram
-
low activity
-
-
?
cucurbitacin E + NAD(P)H
23,24-dihydrocucurbitacin E + NAD(P)+
show the reaction diagram
-
low activity
-
-
?
cucurbitacin E beta-D-glucoside + NAD(P)H
23,24-dihydrocucurbitacin E beta-D-glucoside + NAD(P)+
show the reaction diagram
-
best substrate
-
-
?
cucurbitacin F + NAD(P)H
23,24-dihydrocucurbitacin F + NAD(P)+
show the reaction diagram
-
very low activity
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0604
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.65
-
crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 15000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
5 min, no loss of activity, 10 min, 30% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE