NADH cannot replace NADPH as reductant. The Delta8,9-double bond of (+)-cis-isopulegone and the Delta1,2-double bond of (+/-)-piperitone are not substrates. The enzyme from peppermint (Mentha x piperita) converts (+)-pulegone into both (-)-menthone and (+)-isomenthone at a ratio of 70:30 for native enzyme but it does not catalyse the reverse reaction. This enzyme is a member of the medium-chain dehydrogenase/reductase superfamily.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
(-)-menthone:NADP+ oxidoreductase
NADH cannot replace NADPH as reductant. The Delta8,9-double bond of (+)-cis-isopulegone and the Delta1,2-double bond of (+/-)-piperitone are not substrates. The enzyme from peppermint (Mentha x piperita) converts (+)-pulegone into both (-)-menthone and (+)-isomenthone at a ratio of 70:30 for native enzyme but it does not catalyse the reverse reaction. This enzyme is a member of the medium-chain dehydrogenase/reductase superfamily.
regulation of pulegone metabolism involves menthofuran, the flux of (+)-pulegone through pulegone reductase correlates negatively with the essential oil content of menthofuran, such that menthofuran, and pulegone increase, or decrease, in concert, overview
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio, product analysis by GC-MS, stereospecific reductase
regulation of pulegone metabolism involves menthofuran, the flux of (+)-pulegone through pulegone reductase correlates negatively with the essential oil content of menthofuran, such that menthofuran, and pulegone increase, or decrease, in concert, overview
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
weak competitive inhibition, in peppermint plants grown under low-light conditions, (+)-menthofuran is selectively retained in secretory cells and accumulated to very high levels of up to 20 mM, whereas under regular growth conditions, (+)-menthofuran levels remain very low, below 0.4 mM
although (+)-menthofuran does not inhibit (+)-pulegone reductase activity, stem feeding with menthofuran selectively decreases enzyme transcript levels in immature leaves, thereby accounting for decreased reductase activity and increased pulegone content, overview, the flux of (+)-pulegone through pulegone reductase correlates negatively with the essential oil content of menthofuran, such that menthofuran, and pulegone increase, or decrease, in concert, overview
Keyuan-1 remains a higher pulegone reductase activity, while that of wild-type seedlings is severely decreased, which declined to the 17.71% of the control
p-menthane monoterpene biosynthesis in peppermint glandular trichomes, generation of a mathematical model of peppermint essential oil biosynthesis, monoterpene contents in the essential oil, overview
the Pr activity determines the critical intermediate step to process pulegone and increase essential oil content of the derived products menthone and menthol. Salt-tolerant peppermint Keyuan-1 cultivar maintains normal growth and keeps higher yield and content of essential oils under NaCl stress than wild-type peppermint, but shows no obvious changes in essential oil composition and transcriptional level of enzymes related to essential oil metabolism. The activity of pulegone reductase in salt-tolerant peppermint Keyuan-1 peppermint cultivar preserves the higher amount of menthol and menthone as well as the lower content of menthofuran upon the 150-mM NaCl stress. MAPK-dependent regulation mechanism of essential oil biosynthesis in the salttolerant peppermint
simulation of the effects of environmental stress conditions on essential oil profiles, glandular trichome dynamics, overview. Substantially higher proportion of large-sized glandular trichomes, fewer medium-sized trichomes, and no small trichomes on plants lead to decreased (+)-pulegone and increased (-)-menthone contents
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
neither protease inhibitors nor flavins improve the activity or stability of the purified native enzyme, and the addition of glycerol to the buffers leads to rapid loss of activity upon storage
recombinant enzyme from Escherichia coli strain BL21(DE3), native enzyme by oil gland secretory cell isolation procedure, two steps of anion exchange chromatography, hydroxyapatite chromatography, and beta-NADPH affinity chromatography, the activity is rapidly lost during purification on each matrix, 10fold purification accompanied by loss of over 98% of starting activity
Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase
Ringer, K.L.; McConkey, M.E.; Davis, E.M.; Rushing, G.W.; Croteau, R.
Monoterpene double-bond reductases of the (-)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (-)-isopiperitenone reductase and (+)-pulegone reductase of peppermint
Mathematical modeling-guided evaluation of biochemical, developmental, environmental, and genotypic determinants of essential oil composition and yield in peppermint leaves