Information on EC 1.3.1.97 - botryococcene synthase

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The expected taxonomic range for this enzyme is: Botryococcus braunii

EC NUMBER
COMMENTARY hide
1.3.1.97
-
RECOMMENDED NAME
GeneOntology No.
botryococcene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
C30 botryococcene + NADP+ + diphosphate = presqualene diphosphate + NADPH + H+
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
botryococcenes and methylated squalene biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
C30 botryococcene:NADP+ oxidoreductase
Isolated from the green alga Botryococcus braunii BOT22. Acts in the reverse direction. Involved in the production of botryococcenes, which are triterpenoid hydrocarbons of isoprenoid origin produced in large amount by this alga.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
presqualene diphosphate + NADPH + H+
C30 botryococcene + NADP+ + diphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required for activity
additional information
-
no enzyme activity is evident with Co2+ or Mn2+ as a cofactor
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
squalestatin
-
-
Triton X-100
-
-
Tween 80
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enzyme activity is completely abolished by 0.5% (v/v) Tween 80
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000001
squalestatin
-
at pH 7.3 and 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
in MOPS buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Saccharomyces cerevisiae strain TN7
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression is preferential during rapid growth
gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period