Isolated from the green alga Botryococcus braunii BOT22. Acts in the reverse direction. Involved in the production of botryococcenes, which are triterpenoid hydrocarbons of isoprenoid origin produced in large amount by this alga.
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SYSTEMATIC NAME
IUBMB Comments
C30 botryococcene:NADP+ oxidoreductase
Isolated from the green alga Botryococcus braunii BOT22. Acts in the reverse direction. Involved in the production of botryococcenes, which are triterpenoid hydrocarbons of isoprenoid origin produced in large amount by this alga.
proposed catalytic cascades for the enzyme-mediated biosynthesis of squalene and botryococcene, and molecular modeling of Botryococcus braunii botryococcene and squalene synthase enzymes, overview. Substrate docking and molecular modeling
site-directed mutagenesis, combined N171A and G207Q mutations in the SSL-3 backbone result in complete conversion of SSL-3 from an enzyme that used presqualene diphosphate for botryococcene biosynthesis to one that used presqualene diphosphate for squalene biosynthesis
generation of an expression system for production of high levels of botryococcene in Nicotiana tabacum strain KY 1068 plants by diverting carbon flux from the cytosolic mevalonate pathway or the plastidic methylerythritol phosphate pathway by the targeted overexpression of an avian farnesyl diphosphate synthase along with botryococcene synthase SSL-3 and presqualene diphosphate synthase SSL-1, overview. The structure of purified botryococcene from tobacco is determined by 1H-NMR and 13C-NMR spectral analyses. Modifications of botryococcene in tobacco by coexpressed triterpene methyltransferases (TMTs) from Botryococcus braunii
gene SSL-3, recombinant expression of the enzyme in Nicotiana tabacum, ene constructs consisted of a peptide fusion of SSL-1 and SSL-3 connected by a triplet repeat peptide linker of Gly-Gly-Ser-Gly, with or without appending the C-terminal end (71 amino acids) of the Botryococcus braunii squalene synthase onto the C-terminus of SSL-3 and the FPS gene. The chimeric SSL1-3 genes and FPS gene are inserted downstream of the strong constitutive promoters Pcv and Pca, respectively. For trichome-specific expression of triterpene biosynthesis, the trichome-specific promoters Pcbt and Pcyp16 are fused to 5' end of botryococcene synthase gene, presqualene diphosphate synthase gene, and the FPS gene, respectively
gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period
gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period
gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period
an expression system for production of high levels of botryococcene in Nicotiana tabacum plants can be used involving the recombinant chimeric enzyme, trichome-specific expression of botryococcene metabolism, overview
Bell, S.A.; Niehaus, T.D.; Nybo, S.E.; Chappell, J.
Structure-function mapping of key determinants for hydrocarbon biosynthesis by squalene and squalene synthase-like enzymes from the green alga Botryococcus braunii race B