Information on EC 1.3.1.B13 - polyprenol reductase (NADPH)

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The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
1.3.1.B13
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
polyprenol reductase (NADPH)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an alpha-reduced dolichol + NADP+ = a polyprenol + NADPH + H+
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
dolichol:NADP+ alpha-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
mutations in the SRD5A3 gene results in mental retardation, ophthalmologic and cerebellar defects of the patients
metabolism
the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a polyprenol + NADPH + H+
an alpha-reduced dolichol + NADP+
show the reaction diagram
the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis
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-
?
an alpha-reduced polyprenol + NADPH + H+
a dolichol + NADP+
show the reaction diagram
reduction of the alpha-isoprene subunit of polyprenol
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a polyprenol + NADPH + H+
an alpha-reduced dolichol + NADP+
show the reaction diagram
Q9H8P0
the enzyme (SRD5A3) is necessary for the reduction of the alpha-isoprene unit of polyprenols to form alpha-reduced dolichols, required for synthesis of dolichol-linked monosaccharides, and the oligosaccharide precursor used for N-glycosylation. The presence of residual dolichol in cells depleted for this enzyme suggests the existence of an unexpected alternative pathway for dolichol de novo biosynthesis
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in HEK293T cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H296G
inactive mutant enzyme, shows no reduction of polyprenol