Information on EC 1.4.1.12 - 2,4-diaminopentanoate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.1.12
-
RECOMMENDED NAME
GeneOntology No.
2,4-diaminopentanoate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,4-diaminopentanoate + H2O + NAD(P)+ = 2-amino-4-oxopentanoate + NH3 + NAD(P)H + H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-ornithine degradation II (Stickland reaction)
-
-
urea cycle
-
-
Lysine degradation
-
-
Arginine and proline metabolism
-
-
D-Arginine and D-ornithine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
2,4-diaminopentanoate:NAD(P)+ oxidoreductase (deaminating)
Also acts, more slowly, on 2,5-diaminohexanoate forming 2-amino-5-oxohexanoate, which then cyclizes non-enzymically to 1-pyrroline-2-methyl-5-carboxylate.
CAS REGISTRY NUMBER
COMMENTARY hide
39346-26-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
M-E, SB4
-
-
Manually annotated by BRENDA team
M-E, SB4
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-diaminopentanoate + H2O + NAD(P)+
2-amino-4-oxopentanoate + NH3 + NAD(P)H
show the reaction diagram
2,4-diaminopentanoate + H2O + NAD+
2-amino-4-oxopentanoate + NH3 + NADH
show the reaction diagram
2,4-diaminopentanoate + H2O + NADP+
2-amino-4-oxopentanoate + NH3 + NADPH
show the reaction diagram
-
enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec)
-
-
?
2,5-diaminohexanoate + H2O + NAD(P)+
2-amino-5-oxohexanoate + NH3 + NAD(P)H
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,4-diaminopentanoate + H2O + NAD(P)+
2-amino-4-oxopentanoate + NH3 + NAD(P)H
show the reaction diagram
2,5-diaminohexanoate + H2O + NAD(P)+
2-amino-5-oxohexanoate + NH3 + NAD(P)H
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
pyridoxal 5'-phosphate
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no stimulation of activity by Mg2+, Zn2+, Mn2+, Fe2+, Cd2+, Ni2+, Cu2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-amino-4-ketopentanoate
-
70% inhibition in the presence of 1 mM 2-amino-4-ketopentanoate
5,5'-dithiobis(2-nitrobenzoate)
-
-
acetyl-CoA
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30% inhibition in the presence of 1 mM acetyl-CoA
D-alanine
-
45% inhibition in the presence of 1 mM D-alanine
iodoacetamide
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N-ethylmaleimide
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-
Organic mercurials
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-
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p-chloromercuribenzoate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 1.8
2,4-Diaminopentanoate
-
-
2.5
2,5-Diaminohexanoate
-
-
0.6 - 2100
NAD+
0.11 - 2
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
34
NAD+
Clostridium sticklandii
-
-
5
NADP+
Clostridium sticklandii
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-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016
NAD+
Clostridium sticklandii
-
-
7
2.56
NADP+
Clostridium sticklandii
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-
10
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
-
2,4-diaminopentanoate + NAD+ + H2O
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10
-
half maximal activity at pH 8.0 and 10.0, 2,4-diaminopentanoate + NAD+ + H2O
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay carried out at room temperature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
SDS-PAGE
38930
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calculated from cDNA
72000 - 80000
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sedimentation equilibrium centrifugation
80000
-
gel electrophoresis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 35400 SDS-PAGE, sedimentation centrifugation in 6 M guanidine-HCl; 2 * 40000 SDS-PAGE, gel electrophoresis
homodimer
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gel filtration, 2 * 38000 Da
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
unstable above
391436
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, phosphate buffer, pH 7,5, 10-20% glycerol, one month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE