Information on EC 1.4.1.17 - N-methylalanine dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas sp.

EC NUMBER
COMMENTARY hide
1.4.1.17
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RECOMMENDED NAME
GeneOntology No.
N-methylalanine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-methyl-L-alanine + H2O + NADP+ = pyruvate + methylamine + NADPH + H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
N-methyl-L-alanine:NADP+ oxidoreductase (demethylating, deaminating)
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CAS REGISTRY NUMBER
COMMENTARY hide
56379-51-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MS ATCC 25262
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
methylamine + alpha-ketobutyrate + NADPH
glutamic acid + H2O + NADP+
show the reaction diagram
-
14% of activity with pyruvate
-
-
?
methylamine + oxaloacetate + NADPH
aspartic acid + H2O + NADP+
show the reaction diagram
-
64% of activity with pyruvate
-
-
?
pyruvate + methylamine + NADPH
N-methyl-L-alanine + H2O + NADP+
show the reaction diagram
-
reverse reaction only to limited extend at very low rate
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyruvate + methylamine + NADPH
N-methyl-L-alanine + H2O + NADP+
show the reaction diagram
-
reverse reaction only to limited extend at very low rate
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
substrate inhibition above 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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required for full activity
dithiothreitol
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can replace 2-mercaptoethanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
75
methylamine
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-
0.035
NADPH
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15
pyruvate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.37
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2 - 8.6
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-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36500
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SDS-PAGE
77000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 36500, SDS-PAGE, gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in the absence of 2-mercaptoethanol or dithiothreitol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE