Information on EC 1.5.3.2 - N-methyl-L-amino-acid oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.5.3.2
-
RECOMMENDED NAME
GeneOntology No.
N-methyl-L-amino-acid oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an N-methyl-L-amino acid + H2O + O2 = an L-amino acid + formaldehyde + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
demethylation
-
-
-
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oxidation
-
-
-
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redox reaction
-
-
-
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reduction
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-methyl-L-amino-acid:oxygen oxidoreductase (demethylating)
A flavoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-23-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-N-methyl-L-histidine + O2 + H2O
L-histidine + formaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
alpha-N-methyl-L-tryptophan + O2 + H2O
L-tryptophan + formaldehyde + H2O2
show the reaction diagram
-
alpha-N-methyl-L-tryptophan is identical with L-abrine
-
?
N-methyl-L-amino acid + O2 + H2O
L-amino acid + formaldehyde + H2O2
show the reaction diagram
-
enzyme requires the presence of a free alpha-carboxyl group in the substrate molecule, substrates with aromatic or heterocyclic substituents are more readily oxidized, followed by aliphatic N-methylamino acids with long side chain, more slowly activity towards N-methyl derivatives of beta-hydroxy-amino acids and of basic and acidic amino acids with exception of histidin, very slow demethylation of phenylsarcosine
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?
N-methyl-L-norleucine + O2 + H2O
L-norleucine + formaldehyde + H2O2
show the reaction diagram
-
-
-
?
N-methyl-L-phenylalanine + O2 + H2O
L-phenylalanine + formaldehyde + H2O2
show the reaction diagram
-
-
-
-
?
N-methyl-L-tyrosine + O2 + H2O
L-tyrosine + formaldehyde + H2O2
show the reaction diagram
-
N-methyl-DL-tyrosine is identical with surinamine
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-
?
N-methyldihydroxyphenyl-DL-alanine + O2 + H2O
formaldehyde + dihydroxyphenyl-DL-alanine + H2O2
show the reaction diagram
-
-
-
-
?
N-methylvanillyl-L-alanine + O2 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
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more substrates, not with: N-dimethyl-L-amino acid, N-methyl-D-amino acid, L-amino acid, D-amino acid
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
as prosthetic group
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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not inhibited by 0.01 M benzoate, 0.01 M quinine HCl, 0.01 M atabrine, 0.01 M KCN
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.262
alpha-N-methyl-L-tryptophan
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE