Information on EC 1.5.7.1 - methylenetetrahydrofolate reductase (ferredoxin)

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The expected taxonomic range for this enzyme is: Clostridium formicaceticum

EC NUMBER
COMMENTARY hide
1.5.7.1
-
RECOMMENDED NAME
GeneOntology No.
methylenetetrahydrofolate reductase (ferredoxin)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-methyltetrahydrofolate + 2 oxidized ferredoxin = 5,10-methylenetetrahydrofolate + 2 reduced ferredoxin + 2 H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
folate transformations I
-
-
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)
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-
reductive acetyl coenzyme A pathway
-
-
One carbon pool by folate
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-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
5-methyltetrahydrofolate:ferredoxin oxidoreductase
An iron-sulfur flavoprotein that also contains zinc. The enzyme from Clostridium formicoaceticum catalyses the reduction of methylene blue, menadione, benzyl viologen, rubredoxin or FAD with 5-methyltetrahydrofolate and the oxidation of reduced ferredoxin or FADH2 with 5,10-methylenetetrahydrofolate. However, unlike EC 1.5.1.20, methylenetetrahydrofolate reductase [NAD(P)H], there is no activity with NAD(P)H.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-69-7
cf. EC 1.7.99.5
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + FADH2
5,10-methylenetetrahydrofolate + FAD
show the reaction diagram
-
-
-
-
?
5,10-methylenetetrahydrofolate + reduced ferredoxin
5-methyltetrahydrofolate + ferredoxin
show the reaction diagram
-
-
-
-
?
5-methyltetrahydrofolate + benzyl viologen
5,10-methylenetetrahydrofolate + reduced benzyl viologen
show the reaction diagram
5-methyltetrahydrofolate + FAD
5,10-methylenetetrahydrofolate + FADH2
show the reaction diagram
-
11% of the activity with methylene blue
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-
?
5-methyltetrahydrofolate + menadione
5,10-methylenetetrahydrofolate + reduced menadiol
show the reaction diagram
-
25% of the activity with methylene blue
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-
?
5-methyltetrahydrofolate + rubredoxin
5,10-methylenetetrahydrofolate + reduced rubredoxin
show the reaction diagram
-
11% of the activity with methylene blue
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-
?
additional information
?
-
-
no activity with pyridine nucleotides
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
contains 1.7 FAD per 237000 Da enzyme
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
iron-sulfur protein, contains 15.2 iron and 19.5 acid labile sulfide per 237000 Da enzyme
Zinc
-
enzyme contains 2.3 zinc per 237000 Da enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
5-methyltetrahydrofolate
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-
11.1
benzyl viologen
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
237000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
-
alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 7.6
-
the enzyme is more stable in Tris/HCl than in triethanolamine/HCl or phosphate buffer
437714
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme loses 50% of the activity within 2 h in aerobic buffer (0.1 M Tris/HCl buffer, pH 7.4) with or without a reducing agent and in anaerobic buffer without a reducing agent
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437714
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
10°C, pH 7.4, 50 mM Tris/HCl containing 20% glycerol and 2 mM dithionite in an anaerobic chamber
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE