Information on EC 1.5.8.1 - dimethylamine dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.5.8.1
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RECOMMENDED NAME
GeneOntology No.
dimethylamine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylamine + H2O + electron-transfer flavoprotein = methylamine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methane metabolism
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Methane metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
dimethylamine:electron-transferring flavoprotein oxidoreductase
Contains FAD and a [4Fe-4S] cluster.
CAS REGISTRY NUMBER
COMMENTARY hide
172522-63-3
dehydrogenase, dimethylamine (Hyphomicrobium strain X clone pUHA65/pUHB49 gene dmd precursor)
172522-64-4
dehydrogenase, dimethylamine (Hyphomicrobium strain X clone pUHA65/pUHB49 gene dmd)
68247-64-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Pseudomonas putida ATCC 12633
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diethylamine + H2O + acceptor
ethylamine + acetaldehyde + reduced acceptor
show the reaction diagram
dimethylamine + H2O + 2,6-dichlorophenolindophenol
methylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
dimethylamine + H2O + acceptor
methylamine + formaldehyde + reduced acceptor
show the reaction diagram
dimethylamine + H2O + electron-transfer flavoprotein
methylamine + formaldehyde + reduced electron-transferring flavoprotein
show the reaction diagram
dimethylamine + H2O + electron-transferring flavoprotein
methylamine + formaldehyde + reduced electron-transferring flavoprotein
show the reaction diagram
ethylpropylamine + H2O + acceptor
?
show the reaction diagram
methylethanolamine + H2O + acceptor
?
show the reaction diagram
methylethylamine + H2O + acceptor
ethylamine + formaldehyde + reduced acceptor
show the reaction diagram
methylpropylamine + H2O + acceptor
propylamine + formaldehyde + reduced acceptor
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylamine + H2O + electron-transfer flavoprotein
methylamine + formaldehyde + reduced electron-transferring flavoprotein
show the reaction diagram
dimethylamine + H2O + electron-transferring flavoprotein
methylamine + formaldehyde + reduced electron-transferring flavoprotein
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
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6-S-Cysteinyl-FMN
electron-transferring flavoprotein
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additional information
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independent of NADPH
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Dimethylamine
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10 mM, 23% inhibition
methylamine
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above 6 mM, slight inhibition
phenazine methosulfate
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serves as electron acceptor, maximal activity at 2.4 mM. Above 2.4 mM inhibition
trimethylamine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0156
Dimethylamine
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0.11
N,N,N,N-tetramethyl-o-phenylenediamine
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1.18
phenazine methosulfate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
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dimethylamine dehydrogenase activity under anaerobic growth conditions
95
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dimethylamine monooxygenase activity under aerobic growth conditions
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
7.7
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
138000
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gel filtration
176000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing in liquid nitrogen results in a complete loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 20% v/v ethylene glycol, stable for at least 3 months
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0°C, 3 months without stabilizer, 50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
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