Information on EC 1.5.99.14 - 6-hydroxypseudooxynicotine dehydrogenase

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The expected taxonomic range for this enzyme is: Arthrobacter

EC NUMBER
COMMENTARY hide
1.5.99.14
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RECOMMENDED NAME
GeneOntology No.
6-hydroxypseudooxynicotine dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O = 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nicotine degradation I (pyridine pathway)
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Nicotinate and nicotinamide metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one:acceptor 6-oxidoreductase (hydroxylating)
Contains a cytidylyl molybdenum cofactor [3]. The enzyme, which participates in the nicotine degradation pathway, has been characterized from the soil bacterium Arthrobacter nicotinovorans [1,2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
encoded on megaplasmid pAO1
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O
1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme
molybdopterin cytosine dinucleotide
cofactor is required for assembly of the heterotrimeric molybdenum enzyme
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme
Molybdenum
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contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28.3
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37°C, pH 7.5
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotrimer
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1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE