Information on EC 1.7.1.12 - N-hydroxy-2-acetamidofluorene reductase

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The expected taxonomic range for this enzyme is: Oryctolagus cuniculus

EC NUMBER
COMMENTARY hide
1.7.1.12
-
RECOMMENDED NAME
GeneOntology No.
N-hydroxy-2-acetamidofluorene reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-acetamidofluorene + NAD(P)+ + H2O = N-hydroxy-2-acetamidofluorene + NAD(P)H + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2-acetamidofluorene:NAD(P)+ oxidoreductase
Also acts, more slowly, on N-hydroxy-4-acetamidobiphenyl.
CAS REGISTRY NUMBER
COMMENTARY hide
99890-08-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-hydroxy-2-acetamidofluorene + NAD(P)H
2-acetamidofluorene + NAD(P)+ + H2O
show the reaction diagram
-
other electron donors: cysteine, glutathione, dithiothreitol, 2-mercaptoethanol
-
?
N-hydroxy-4-acetylaminobiphenyl + NAD(P)H
4-acetylaminobiphenyl + NAD(P)+ + H2O
show the reaction diagram
-
much slower reaction compared to N-hydroxy-2-acetamidofluorene
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CuSO4
-
1 mM, 52% inhibition
Disulfiram
-
0.1 mM, 56% inhibition
N-ethylmaleimide
-
1 mM, 86% inhibition
p-chloromercuribenzoate
-
0.2 mM, 81% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
enhances activity, can replace NADH
cysteine
-
enhances activity, can replace NADH
dithiothreitol
-
enhances activity, can replace NADH
glutathione
-
enhances activity, can replace NADH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
low amounts
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 34000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE