Information on EC 1.7.1.2 - Nitrate reductase [NAD(P)H]

Word Map on EC 1.7.1.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.7.1.2
-
RECOMMENDED NAME
GeneOntology No.
Nitrate reductase [NAD(P)H]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nitrite + NAD(P)+ + H2O = nitrate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Nitrogen metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
nitrite:NAD(P)+ oxidoreductase
An iron-sulfur molybdenum flavoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-27-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ankistrodesmus braunii
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Chlorella variegata
-
-
-
Manually annotated by BRENDA team
alga
-
-
Manually annotated by BRENDA team
barley, mutant nar la
-
-
Manually annotated by BRENDA team
strain KT2442
UniProt
Manually annotated by BRENDA team
strain KT2442
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Triticale sp.
Triticum sativum x Secale cereale, winter triticale
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hexahydro-1,3,5-trinitro-1,3,5-triazine + NADPH
hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine + NADP+
show the reaction diagram
-
-
hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine is the first reduced product formed by a two-electron reduction mechanism. Subsequent reduction of hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine followed by rapid ring cleavage gives methylenedinitramine which in turn decomposes in water to N2O, NH4+ and HCHO
-
?
NAD(P)H + H+ + nitrate
NAD(P)+ + nitrite + H2O
show the reaction diagram
Triticale sp.
-
-
-
-
?
NAD(P)H + nitrate
?
show the reaction diagram
-
plays a role in first step of nitrate assimilation
-
-
-
NAD(P)H + nitrate
NAD(P)+ + nitrite + H2O
show the reaction diagram
NADPH + nitrate
NADP+ + nitrite
show the reaction diagram
-
-
-
-
?
nitrate + NAD(P)H
nitrite + NAD(P)+ + H2O
show the reaction diagram
reduced methyl viologen + nitrate
oxidized methyl viologen + nitrite + H2O
show the reaction diagram
Triticale sp.
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NAD(P)H + nitrate
?
show the reaction diagram
-
plays a role in first step of nitrate assimilation
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome b557
-
NAD(P)H
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Molybdenum
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
azide
cyanide
Dithionite
-
-
NAD(P)H
p-chloromercuribenzoate
p-hydroxymercuribenzoate
Ankistrodesmus braunii
-
-
additional information
-
insensitive to 1 mM tungsten
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000004
FAD
Ankistrodesmus braunii
-
as a protector of NADH-cytochrome c reductase activity
0.0115 - 0.16
NADH
0.0145 - 0.045
NADPH
0.11 - 0.74
nitrate
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
470
NADPH
Ankistrodesmus braunii
-
-
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.044
azide
-
at low concentration
0.0094
cyanide
-
at low concentration
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000013
Triticale sp.
-
crude extract, using FMNH as electron donor, in 50 mM Tris-HCl buffer, pH 7.4, at 25C
0.0000167
Triticale sp.
-
crude extract, using methyl viologen as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.7, at 25C
0.000117
Triticale sp.
-
crude extract, using NADPH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25C
0.000184
Triticale sp.
-
crude extract, using NADH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25C
0.455
Triticale sp.
-
after 2481.9fold purification, using NADH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25C
0.718
Chlorella variegata
-
NADH-nitrate reductase
0.766
Chlorella variegata
-
NADPH-nitrate reductase
1.3
-
after purification
61.25
Ankistrodesmus braunii
-
-
72 - 80
Ankistrodesmus braunii
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 7.7
Chlorella variegata
-
-
7.8 - 8.2
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
at pH 7 and 9: about 60% of activity maximum
7.1 - 8.2
Chlorella variegata
-
at pH 7.1 and 8.2: about 50% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
activity is maximal at early stages of growth, 30 days after sowing, then reaching to almost zero at 90 days after sowing
Manually annotated by BRENDA team
Triticale sp.
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
specifically located in the pyrenoid region of chloroplast
Manually annotated by BRENDA team
additional information
-
not membrane-bound
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
-
SDS-PAGE
190000
Triticale sp.
-
SDS-PAGE
230000
-
Rhodotorula glutinis, sucrose density gradient centrifugation, gel filtration
365000
-
Candida nitratophila, sedimentation equilibrium centrifugation
460000 - 467400
Ankistrodesmus braunii
-
Ankistrodesmus braunii, gel filtration, density gradient centrifugation, disc gel electrophoresis, pore gradient electrophoresis, sucrose-density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
Triticale sp.
-
x * 190000, SDS-PAGE
dimer
-
2 * 118000, Rhodotorula glutinis, SDS-PAGE
homodimer
-
2 * 65000, SDS-PAGE
octamer
Ankistrodesmus braunii
-
8 * 58750-59000, Ankistrodesmus braunii, SDS-PAGE
tetramer
-
4 * 95000, Candida nitratophila, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
inactivation
60
-
3 min, complete loss of NADPH-nitrate reductase activity
additional information
-
heat activation of benzyl viologen:nitrate reductase activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Labile to dilution with protein-free buffer, 2.5 mg bovine serum albumin, stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 50% loss of activity after 7 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and blue-dextran-Sepharose column chromatography
Triticale sp.
-
DEAE-Toyopearl 650M column chromatography, Sephacryl S-200 gel filtration and DEAE-cellulose DE-52 column chromatography
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
environmental protection
-
hexahydro-1,3,5-trinitro-1,3,5-triazine is widely used for military and commercial purposes due to its high explosive properties. Hexahydro-1,3,5-trinitro-1,3,5-triazine and its degradation products are toxic, mutagenic and carcinogenic to humans and other biological systems. The biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine by NAD(P)H nitrate reductase from Aspergillus niger under anaerobic conditions