Information on EC 2.1.1.141 - jasmonate O-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.141
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RECOMMENDED NAME
GeneOntology No.
jasmonate O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + jasmonate = S-adenosyl-L-homocysteine + methyl jasmonate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alpha-Linolenic acid metabolism
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:jasmonate O-methyltransferase
9,10-Dihydrojasmonic acid is a poor substrate for the enzyme. The enzyme does not convert 12-oxo-phytodienoic acid (a precursor of jasmonic acid), salicylic acid, benzoic acid, linolenic acid or cinnamic acid into their corresponding methyl esters. Enzyme activity is inhibited by the presence of divalent cations, e.g., Ca2+, Cu2+, Mg2+ and Zn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
346420-58-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
susp. vaseyana
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cultivar Elsanta
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
black cottonwood
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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overexpression of enzyme in the Glycine max seeds results in decreased amounts of tryptophan, palmitic acid, linolenic acid, and stachyose, but increased levels of gadoleic acid and genistein. In particular, seeds contain 120.0-130.5% more genistein and 60.5-82.1% less stachyose than the wild type
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 9,10-dihydrojasmonic acid
S-adenosyl-L-homocysteine + ?
show the reaction diagram
poor substrate
-
-
?
S-adenosyl-L-methionine + benzoate
S-adenosyl-L-homocysteine + methyl benzoate
show the reaction diagram
22% activity compared to jasmonate
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-
?
S-adenosyl-L-methionine + jasmonate
S-adenosyl-L-homocysteine + methyl jasmonate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + jasmonate
S-adenosyl-L-homocysteine + methyl jasmonate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
significantly stimulates enzyme activity (to about 250%)
Na+
has a mild stimulation on enzyme activity (to about 160%)
NH4+
has a mild stimulation on enzyme activity (to about 165%)
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe3+
complete inhibition at 5 mM
Na+
-
5 mM, 50-60% inhibition
NH4+
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5 mM, 50-60% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0631 - 0.341
Benzoate
0.0223 - 0.38
jasmonate
0.0063 - 0.0064
S-adenosyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.028
Benzoate
0.013 - 25
jasmonate
70
S-adenosyl-L-methionine
Arabidopsis thaliana
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pH 7.5, 20C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
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as fusion protein with GST
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
at pH 6.0, pH 7.0, pH 8.0 and pH 8.5, the enzyme displays about 80%, 50%, 20% and 5% of its maximal activity, respectively
734870
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 60
the enzyme is 100% stable for 30 min at 4C. It loses about 10%, 20% and 40% of its maximal activity when incubated at 37C, 42C and 50C, respectively for 30 min. When incubated at 60C for 30 min, the enzyme loses all of its activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Brassica campestris subsp. Pekinensis
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expressed in Escherichia coli
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expressed in Escherichia coli BL21 (DE3) pLysS cells and Saccharomyces cerevisiae strain INV Sc1
expressed in Glycine max seeds
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expressed in Nicotiana tabacum leaves
expression in Escherichia coli
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gene JMT, overexpression in Panax ginseng roots via Agrobacterium tumefaciens strain C58C1 transfection
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gene JMT, overexpression under control of CaMV 35S promoter in Solanum tuberosum cv. Jopung tubers via Agrobacterium tumefaciens transfection
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NTR1 gene, functional overexpression in transgenic Glycine max plants under control of the CaMV 35S promoter using the Agrobacterium tumefaciens, strain LBA4404, transfection method
transgenic Arabidopsis overexpressing the JMT gene exhibit significantly high degree of resistance against the infection with virulent pathogen Pseudomonas syringae pv tomato
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is up-regulated in response to infestation with the brown planthopper Nilaparvata lugens
hexanoic acid induces the expression of the enzyme
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the enzyme expression is induced by methyl jasmonate and salicylic acid as well as alamethicin
the enzyme's transcript levels decrease steadily during strawberry fruit ripening
treatment with salicylic acid slightly decreases enzyme expression 24 h after treatment
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
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expression of the enzyme in transgenic Chinese cabbage plants provides high levels of field resistance against Erwinia carotovora
L245DEL
N361S
the mutation leads to higher specific activity with benzoate than with jasmonate
S153Y
the mutation leads to strongly reduced activity with benzoate or jasmonate
S153Y/N361S
the mutation leads to strongly reduced activity with benzoate or jasmonate
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
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genetic introduction of methyljasmonate-producing gene can be used to achieve fortified resistance of plants against various pathogens