Information on EC 2.1.1.162 - glycine/sarcosine/dimethylglycine N-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Methanohalophilus portucalensis

EC NUMBER
COMMENTARY hide
2.1.1.162
-
RECOMMENDED NAME
GeneOntology No.
glycine/sarcosine/dimethylglycine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3 S-adenosyl-L-methionine + glycine = 3 S-adenosyl-L-homocysteine + betaine
show the reaction diagram
S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine
show the reaction diagram
S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine
show the reaction diagram
S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycine betaine biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:glycine(or sarcosine or N,N-dimethylglycine) N-methyltransferase [sarcosine(or N,N-dimethylglycine or betaine)-forming]
Unlike EC 2.1.1.156 (glycine/sarcosine N-methyltransferase), EC 2.1.1.157 (sarcosine/dimethylglycine N-methyltransferase) and EC 2.1.1.161 (dimethylglycine N-methyltransferase), this enzyme, from the halophilic methanoarchaeon Methanohalophilus portucalensis, can methylate glycine and all of its intermediates to form the compatible solute betaine [1].
CAS REGISTRY NUMBER
COMMENTARY hide
260415-20-1
-
294211-11-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; strain FDF1, two different peaks showing GSDMT activity eluted from chromatofocusing column
-
-
Manually annotated by BRENDA team
; strain FDF1, two different peaks showing GSDMT activity eluted from chromatofocusing column
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + glycine
S-adenosyl-L-homocysteine + betaine
show the reaction diagram
-
(overall reaction)
-
-
?
S-adenosyl-L-methionine + glycine
S-adenosyl-L-homocysteine + N-methylglycine
show the reaction diagram
-
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
-
-
?
S-adenosyl-L-methionine + glycine
S-adenosyl-L-homocysteine + sarcosine
show the reaction diagram
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + betaine
show the reaction diagram
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
show the reaction diagram
-
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
-
-
?
S-adenosyl-L-methionine + N-methylglycine
S-adenosyl-L-homocysteine + N,N-dimethylglycine
show the reaction diagram
-
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
-
-
?
S-adenosyl-L-methionine + sarcosine
S-adenosyl-L-homocysteine + N,N-dimethylglycine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + glycine
S-adenosyl-L-homocysteine + N-methylglycine
show the reaction diagram
-
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
-
-
?
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
show the reaction diagram
-
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
-
-
?
S-adenosyl-L-methionine + N-methylglycine
S-adenosyl-L-homocysteine + N,N-dimethylglycine
show the reaction diagram
-
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
two different proteins eluted from chromatofocusing column are differently activated by addition of K+, the peak D protein exhibits low GSDMT activity only at K+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
-
complete inhibition above 0.6 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
glycine
-
pH 7.2, 37C
2.45
N,N-dimethylglycine
-
pH 7.2, 37C
3.14
N-methylglycine
-
pH 7.2, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
N,N-dimethylglycine as substrate, pH 7.2, 37C
0.0037
-
glycine as substrate, pH 7.2, 37C
0.0046
-
N-methylglycine as substrate, pH 7.2, 37C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
-
glycine as subtrate
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4
-
alpha-subunit, 2-D gel electrophoresis
4.8
-
beta-subunit, 2-D gel electrophoresis
5
-
gamma-subunit, 2-D gel electrophoresis
5.2
-
delta-subunit, 2-D gel electrophoresis
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE