Information on EC 2.1.1.173 - 23S rRNA (guanine2445-N2)-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.173
-
RECOMMENDED NAME
GeneOntology No.
23S rRNA (guanine2445-N2)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + guanine2445 in 23S rRNA = S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine2445-N2)-methyltransferase
The enzyme methylates 23S rRNA in vitro, assembled 50S subunits are not a substrate [1]. The enzyme specifically methylates guanine2445 at N2 in 23S rRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-26-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
-
RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
additional information
?
-
P75864
the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Staphylococcus aureus
-
gene rlmKL, DNA and amino acid sequence determination and analysis, gene rlmL/ycbY has been renamed rlmKL
-
gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)
YcbY protein carrying a C-terminal His6 tag is expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Show AA Sequence (520 entries)
Please use the Sequence Search for a certain query.