Information on EC 2.1.1.265 - tellurite methyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.1.1.265
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RECOMMENDED NAME
GeneOntology No.
tellurite methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + tellurite = S-adenosyl-L-homocysteine + methanetelluronate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tellurite methyltransferase
The enzyme is involved in the detoxification of tellurite. It can also methylate selenite and selenium dioxide.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene tehB
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
replacement of the conserved aspartate residue in motif I by asparagine or alanine, or of the conserved phenylalanine in motif II by tyrosine or alanine, decreases resistance to background levels
metabolism
the Escherichia coli chromosomal determinant for tellurite resistance consists of two genes tehA and tehB, which, when expressed on a multicopy plasmid, confer resistance to K2TeO3 at 0.128 mg/ml
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + tellurite
S-adenosyl-L-homocysteine + methanetelluronate
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + tellurite
S-adenosyl-L-homocysteine + methanetelluronate
show the reaction diagram
P25397
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sinefungin
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a cofactor analogue of S-adenosyl-L-methionine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer or dimer
x * 26256, His6-tagged TehB, sequence caculation, x * 27000-28000, recombinant His6-tagged TehB, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TehB complexed with S-adenosyl-L-methionine or sinefungin, hanging drop vapour diffusion method, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, the latter containing 0.1 M NaF and 16-18% PEG 3350, at 20°C, formation of sinefungin complex crystals by microseeding of crystals in reservoir solution containing 5 mM sinefungin, method optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme with a TEV protease cleavage site from Escherichia coli strain BL21(DE3) PlysS by nickel affinity chromatography, cleavage of the His-tag, followed by gel filtration
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recombinant His6-tagged wild-type TehB from Escherichia coli strains C41(DE3) and BL21(DE3), respectively, by nickel affinity chromatographhy
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene tehB, expression as His-tagged enzyme with a TEV protease cleavage site in Escherichia coi strain BL21 (DE3) PlysS
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gene tehB, expression of His6-tagged wild-type enzyme in Escherichia coli strain coli C41(DE3), expression of the mutant TehB enzymes in tehAB operon from pTWT101, a pTZ19R clone, in Escherichia coli strain JM109, expression of His6-tagged mutant enzymes in Escherichia coli strain BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D36A
site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished
D36N
site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished
D59A
site-directed mutagenesis of a motif I residue, the tellurite resistance of the mutant is abolished
D97E
site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is unaltered
D97N
site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is partially reduced
F98A
site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is abolished
F98Y
site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is abolished
Y96A
site-directed mutagenesis of a motif II residue, the tellurite resistance of the mutant is partially reduced
additional information
mutagenesis by the two-stage PCR-based overlapping extension method