Information on EC 2.1.1.266 - 23S rRNA (adenine2030-N6)-methyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.1.1.266
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RECOMMENDED NAME
GeneOntology No.
23S rRNA (adenine2030-N6)-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + adenine2030 in 23S rRNA = S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (adenine2030-N6)-methyltransferase
The recombinant RlmJ protein is most active in methylating deproteinized 23S ribosomal subunit, and does not methylate the completely assembled 50S subunits [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the active site of RlmJ with motif IV sequence 164DPPY167 is more similar to DNA m6A MTases than to RNA m6 2A MTases, and structural comparison suggests that RlmJ binds its substrate base similarly to DNA MTases T4Dam and M.TaqI
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant C-terminally His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.0015 ml of protein solution with an equal amount of reservoir solution containing 0.2 M sodium sulfate decahydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000, and equilibration against 0.08 ml of reservoir solution, 8 days, 20°C, X-ray diffraction strucure determination and analysis at 1.8 A resolution
purified recombinant enzyme in apoform, in complex with the cofactor S-adenosyl-L-methionine, or with product S-adenosyl-L-homocysteine plus substrate analogue AMP, X-ray diffraction structure determination and analysis at 1.85-2.0 A resolution, enzyme apostructure analysis using PDB ID 2OO3, rigid-body refinement of the RlmJAPO structure against the RlmJSAM data
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant wild-type and mutant enzymes
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene rlmJ, sequence comparisons, recombinant expression of wild-type and mutant enzymes
gene yhiR, expression as C-terminally His6-tagged protein in escherichia coli strain AG1
gene yhiR, recombinant expresssion of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D164A
site-directed mutagenesis
H6D
site-directed mutagenesis
K18A
site-directed mutagenesis
K18R
site-directed mutagenesis
Y4A
site-directed mutagenesis
Y4F
site-directed mutagenesis
additional information
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