Information on EC 2.1.1.27 - tyramine N-methyltransferase

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The expected taxonomic range for this enzyme is: Hordeum vulgare

EC NUMBER
COMMENTARY hide
2.1.1.27
-
RECOMMENDED NAME
GeneOntology No.
tyramine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + tyramine = S-adenosyl-L-homocysteine + N-methyltyramine
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tyrosine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tyramine N-methyltransferase
Has some activity on phenylethylamine analogues.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-96-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
L. var. Betzes, barley
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-phenethanolamine + S-adenosyl-L-methionine
?
show the reaction diagram
-
23% of activity with tyramine
-
-
?
p-chloroethylamine + S-adenosyl-L-methionine
?
show the reaction diagram
-
29% of activity with tyramine
-
-
?
S-adenosyl-L-methionine + alpha-methyltyramine
?
show the reaction diagram
-
i.e. paredrine, 53% of activity with tyramine
-
-
?
S-adenosyl-L-methionine + tyramine
N-methyltyramine + S-adenosyl-L-homocysteine
show the reaction diagram
-
conversion rate is higher with (-)-L-diastereoisomer of methionine
-
?
additional information
?
-
-
involved in hordenine biosynthesis
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
involved in hordenine biosynthesis
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactor requirement
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Homotyramine
-
-
N-Methyltyramine
-
-
p-chloromercuribenzoate
-
strong
additional information
-
EDTA is not inhibitory
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
S-adenosyl-L-methionine
-
-
0.02
tyramine
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of seedling
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
heat-labile: 70% loss of activity after 10 min
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
sulfhydryl compound necessary for stabilization
-
441284
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, addition of sucrose to a final concentration of 0.2 M, several weeks
-
4°C, pH 7.0 and 5 mM 2-mercaptoethanol, several days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE