Information on EC 2.1.1.278 - indole-3-acetate O-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.278
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RECOMMENDED NAME
GeneOntology No.
indole-3-acetate O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (indol-3-yl)acetate = S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methyl indole-3-acetate interconversion
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(indol-3-yl)acetate O-methyltransferase
Binds Mg2+. The enzyme is found in plants and is important for regulation of the plant hormone (indol-3-yl)acetate. The product, methyl (indol-3-yl)acetate is inactive as hormone [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene IAMT1
SwissProt
Manually annotated by BRENDA team
clone Nisqually-1
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (indol-3-yl)acetate
S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
show the reaction diagram
S-adenosyl-L-methionine + (indol-3-yl)butyrate
S-adenosyl-L-homocysteine + methyl (indol-3-yl)butyrate
show the reaction diagram
S-adenosyl-L-methionine + dichlorophenoxyacetic acid
S-adenosyl-L-homocysteine + ?
show the reaction diagram
at 5% of the catalytic activity compared to (indol-3-yl)acetate
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (indol-3-yl)acetate
S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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mild activation at 5 mM
Mg2+
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binds Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
5 mM, 15% inhibition
Fe3+
5 mM, more than 95% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013 - 0.0304
(indol-3-yl)acetate
0.0073 - 0.0231
S-adenosyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025 - 0.028
(indol-3-yl)acetate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.15
(indol-3-yl)acetate
Arabidopsis thaliana
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pH 7.5, 25C
6264
4.782
S-adenosyl-L-methionine
Populus trichocarpa
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at pH 7.5 and 25C
24
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
pH 6.5: 20% of maximal activity, pH 9.0: 30% of maximal activity
6.7 - 9
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at pH 6.5, the enzyme has about 34% of its maximal activity, whereas at pH 9.0, the enzyme displays about 40% of the maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 40
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activity at 4C and 40C is about 40% of the maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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low level of expression
Manually annotated by BRENDA team
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highest level of expression
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the crystal structure of the enzyme is determined and refined to 2.75 A resolution
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column agarose chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) CodonPlus cells
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expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
targeted nuclear expression of Triticum aestivum gene TaWRKY71-1, encoding a WRKY transcription factor, in Arabidopsis thaliana induces the expression if gene IAMT1
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information