Information on EC 2.1.1.316 - mitomycin 6-O-methyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces lavendulae

EC NUMBER
COMMENTARY hide
2.1.1.316
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RECOMMENDED NAME
GeneOntology No.
mitomycin 6-O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 6-demethylmitomycin A = S-adenosyl-L-homocysteine + mitomycin A
show the reaction diagram
S-adenosyl-L-methionine + 6-demethylmitomycin B = S-adenosyl-L-homocysteine + mitomycin B
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:6-demethylmitomycin-A 6-O-methyltransferase
The enzyme, characterized from the bacterium Streptomyces lavendulae, is involved in the biosynthesis of the quinone-containing antibiotics mitomycin A and mitomycin B.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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an engineered Streptomyces lavendulae strain in which the mmcR methyltransferase gene has been deleted fails to produce the three mitomycins A, B, and C
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 6-demethylmitomycin A
S-adenosyl-L-homocysteine + mitomycin A
show the reaction diagram
S-adenosyl-L-methionine + 6-demethylmitomycin B
S-adenosyl-L-homocysteine + mitomycin B
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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Mg2+ or Ca2+ do not alter the reactivity of the enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
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completely inhibits the methylation reaction
Fe2+
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4-fold decrease of enzyme activity
Mn2+
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4-fold decrease of enzyme activity
Zn2+
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completely inhibits the methylation reaction
additional information
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increasing the salt concentration from 50 to 500 mM resuls in a gradual decrease in activity to 60% of the initial value
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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presence of 1 mM EDTA results in a slight but significantly higher activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
7-demethylmitomycin A
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pH 7.4, 30°C
0.0038
S-adenosyl-L-methionine
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pH 7.4, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0017
7-demethylmitomycin A
Streptomyces lavendulae
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pH 7.4, 30°C
0.0018
S-adenosyl-L-methionine
Streptomyces lavendulae
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pH 7.4, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
7-demethylmitomycin A
Streptomyces lavendulae
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pH 7.4, 30°C
197627
0.000458
7-demethylmitomycin B
Streptomyces lavendulae
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pH 7.4, 30°C
197863
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, crystal structures of the mitomycin-7-O-methyltransferase–S-adenosylhomocysteine binary complex and mitomycin-7-O-methyltransferase–S-adenosyl-L-homocysteine–mitomycin A ternary complex at resolutions of 1.9 and 2.3 A, respectively
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as N-terminal polyhistidine-tagged protein
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His-tagged MmcR is expressed in Escherichia coli BL21(DE3)
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