Information on EC 2.1.1.34 - tRNA (guanosine18-2'-O)-methyltransferase

Word Map on EC 2.1.1.34
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.34
-
RECOMMENDED NAME
GeneOntology No.
tRNA (guanosine18-2'-O)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanosine18-2'-O)-methyltransferase
The enzyme catalyses the methylation of guanosine18 in tRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-01-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CA274
-
-
Manually annotated by BRENDA team
Marinomonas sp.
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
show the reaction diagram
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
show the reaction diagram
-
involved in post-transcriptional tRNA modification
-
-
-
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
show the reaction diagram
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-AGA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-AGA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-dG18 mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-dG18 mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GAA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GAA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GGA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GGA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GUA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GUA mutant transcript
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + 2-methylthio N6(gamma,gamma,dimethylallyl)adenosine in tRNA
show the reaction diagram
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
show the reaction diagram
S-adenosyl-L-methionine + yeast tRNA-Phe
S-adenosyl-L-homocysteine + yeast tRNA-Phe containing 2'-O-methylguanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
show the reaction diagram
-
involved in post-transcriptional tRNA modification
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
activation, 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DELTAD-arm
-
tRNAPhe variant from yeast with a modification in the D-stem, inhibition
-
G18A
-
tRNAPhe variant from yeast with an exchange of G to A at position 18, inhibition
-
S-adenosyl-L-homocysteine
-
strong, product inhibition, competitive
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
enhances the affinity for tRNA and induces formation of the tetramer structure
spermine
-
activation, 5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000006
Escherichia coli tRNAIle
-
-
-
0.000005
Escherichia coli tRNAMet
-
-
-
0.00001
Escherichia coli tRNAPhe
-
-
-
0.021 - 0.27
guanosine18 in tRNATyr
-
0.00011 - 0.0013
guanosine18 in yeast tRNAPhe
-
0.000082
guanosine18 in yeast tRNAPhe mutant transcript
-
pH and temperature not specified in the publication, Vmax: 3.1 micromol/mg/h
-
0.0125
guanosine18 in yeast tRNAPhe-AGA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.00675 micromol/mg/h
-
0.00082
guanosine18 in yeast tRNAPhe-dG18 mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.1 micromol/mg/h
-
0.0002
guanosine18 in yeast tRNAPhe-GAA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.135 micromol/mg/h
-
0.000245
guanosine18 in yeast tRNAPhe-GGA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 1.2 micromol/mg/h
-
0.0003
guanosine18 in yeast tRNAPhe-GUA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.18 micromol/mg/h
-
0.00047 - 28
S-adenosyl-L-methionine
0.000005 - 0.00001
yeast tRNAPhe
-
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00205
DELTAD-arm
-
-
-
0.00079
G18A
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
45
-
Bacillus subtilis tRNALeu
55
-
Bacillus subtilis tRNAThr
additional information
-
optimal temperature for methylation is ca. 20C lower than the melting temperature of the tRNA, overview
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
-
strain HB 8 shows 80% activity at 40C, strain HB 27 shows 60% activity
52 - 79
-
at 67C content of the TrmH protein is ca. 1/5000 of the total proteins, content of the TrmH protein in 52C cultured cells slightly decreases as compared with that in 67C cultured cells, TrmH contents in cells cultured at 75 or 79C are similar to that at 67C
55 - 68
-
about half-maximal activity at 55C and 68C, yeast tRNAPhe
55 - 75
-
about half-maximal activity at 55C and 75C, Escherichia coli tRNAfMet
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aquifex aeolicus (strain VF5)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
gel filtration
21000
-
gel filtration
54000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by the hanging-drop method, at 1.85 A resolution, diffraction-quality crystals in the pH range 4.04.5, enzyme can not be crystallized with S-adenosyl-L-methionine or an analog in the active site
in complex with S-adenosyl-L-methionine
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
Thermus thermophilus strain HB27: 40% of maximal activity, strain HB8: 80% of maximal activity
60
-
stable below, tRNAPhe
70
-
stable below, tRNAMet
90
-
t1/2: 20 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
urea, stable to 6 M
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30 C, Tris-HCl buffer, pH 7.5, 50% glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on S-adenosyl-L-homocysteine-Sepharose, elution with 6 M urea
-
gel filtration, more than 99% pure
native and recombinant from Escherichia coli
-
wild-type and mutants purified by gel filtration, mutants R11A and R166A purified by repeated ion-exchange chromatography, to homogeneity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence analysis of spoU gene, encodes tRNA (Gm18) 2'-O-methyltransferase
-
expressed in Escherichia coli
-
expression in Escherichia coli with and without His-tag, amino acid sequence analysis and comparison
-
expression inb Escherichia coli
into vector pThioHisB and transformed into Escherichia coli strain DH5alpha, overexpressed in Escherichia coli Rosetta(DE3)/pLysS cells
vector pET30aGm expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D180A
-
42% of wild-type activity
D190A
-
5% of wild-type activity
E124D
-
small increase in Km-value
E124Q
-
no enzymic activity
E182A
-
29% of wild-type activity
H34A
-
no change of affinity for S-adenosyl-L-methionine and tRNA
H71A
-
has increased affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
I142A
-
kinetic parameters comparable to wild-type
K186A
-
7% of wild-type activity
K32A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
K90A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
L101A
-
13fold increase in Km-value
L151A
-
5fold increase in Km-value
L179Stop
M144A
-
15fold increase in Km-value
N152D
-
no enzymic activity
N152E
-
no enzymic activity
N35A
-
marked decrease in methyl transfer activity
N35D
-
marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine
N35Q
-
dramatic increase in Km-value
P143Q
-
small increase in Km-value
Q185A
-
16% of wild-type activity
R109A
-
no change of affinity for S-adenosyl-L-methionine and slightly increased affinity for tRNA
R11A
-
has lost its affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R14M
-
dissociation of subunits consistent with that from the wild-type enzyme
R166A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R168A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R176A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R19A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R41A
-
no enzymic activity
R41K
-
30fold increase in Km-value
R41M
-
large increase in Km-value
R8A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
S150A
-
large increase in Km-value
S150C
-
Km-value similar to wild-type, decrease in Vmax
S150T
-
4fold increase in Km-value
S37G
-
5fold increase in Km-value
T99A
-
small increase in Km-value
V128A
-
small increase in Km-value
V187A
-
11% of wild-type activity
Y184A
-
10% of wild-type activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
Show AA Sequence (1178 entries)
Please use the Sequence Search for a certain query.