Information on EC 2.1.1.38 - O-demethylpuromycin O-methyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces alboniger

EC NUMBER
COMMENTARY hide
2.1.1.38
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RECOMMENDED NAME
GeneOntology No.
O-demethylpuromycin O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + O-demethylpuromycin = S-adenosyl-L-homocysteine + puromycin
show the reaction diagram
puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxyl group with an amino group and acylating this with 4-O-methyltyrosine
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
puromycin biosynthesis
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Puromycin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:O-demethylpuromycin O-methyltransferase
Puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxy group with an amino group and acylating this with 4-O-methyltyrosine.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-04-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N-acetyl-O-demethylpuromycin
S-adenosyl-L-homocysteine + N-acetylpuromycin
show the reaction diagram
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?
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
show the reaction diagram
additional information
?
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L-tyrosine, L-tyrosine ethyl ester, L-tyrosine amide, catechol and epinephrine are not substrates
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucose
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reduced enzyme form, represses puromycin synthesis
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
O-demethylpuromycin
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0.01
S-adenosyl-L-methionine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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broad pH optimum over the range 7.0-9.0 with a maximum at pH 8.0
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
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sucrose density gradient centrifugation
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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very unstable at pH 7.5 and 38C
485314
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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rapidly inactivated at 37C, very unstable even at low temperatures, enzyme activity is destroyed upon boiling
38
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very unstable at 38C and pH 7.5
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilized by addition of S-adenosyl-L-methionine
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-65C, can be stored for several months without loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase, cloning of the complete gene cluster for puromycin and expression in heterologous hosts
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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product puromycin is an antibiotic and antitumor agent