Information on EC 2.1.1.59 - [cytochrome c]-lysine N-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.59
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RECOMMENDED NAME
GeneOntology No.
[cytochrome c]-lysine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + [cytochrome c]-L-lysine = S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lysine degradation
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:[cytochrome c]-L-lysine N6-methyltransferase
One of a group of enzymes methylating proteins; see also EC 2.1.1.43 histone-lysine N-methyltransferase and EC 2.1.1.60 calmodulin-lysine N-methyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
82047-78-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytochrome c L-lysine
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
show the reaction diagram
S-adenosyl-L-methionine + myoglobin L-lysine
S-adenosyl-L-homocysteine + myoglobin N6-methyl-L-lysine
show the reaction diagram
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10% activity compared to cytochrome c
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytochrome c L-lysine
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-Adenosyl-D-homocysteine
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-
S-adenosyl-L-homocysteine
S-adenosyl-L-methionine
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000121
apocytochrome c
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-
0.007 - 0.04
CNBr peptide
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0.03 - 1.7
cytochrome c
0.0095 - 19
S-adenosyl-L-methionine
additional information
additional information
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-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
S-adenosyl-L-methionine
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000117
-
partially purified enzyme
0.000289
-
partially purified enzyme
0.000508
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purified enzyme
0.0295
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purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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13.6% of the enzyme activity
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Manually annotated by BRENDA team
additional information
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overview
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
97000
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gel filtration
120000
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gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 7.0, crude extract supernatant, stable
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0-4°C, 50% saturated ammonium sulfate, stable for several months
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purified enzyme is stabilized by addition of 66% ammonium sulfate during storage
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of gluthathione-S-transferase-tagged Cmt1p in Saccharomyces cerevisiae and of His-tagged in Escherichia coli, Myc-tagged
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in vitro transcription and translation of full length iso-1-cytochrome c gene, serves as substrate
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