Information on EC 2.1.1.60 - calmodulin-lysine N-methyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.1.1.60
-
RECOMMENDED NAME
GeneOntology No.
calmodulin-lysine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + calmodulin L-lysine = S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lysine degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:calmodulin-L-lysine N6-methyltransferase
One of a group of enzymes methylating proteins; see also EC 2.1.1.43 histone-lysine N-methyltransferase and EC 2.1.1.59 [cytochrome-c]-lysine N-methyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
75603-20-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Col-0
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
the enzyme is the major methyltransferase of calmodulin in human cells and a Hsp90 client protein
physiological function
-
the enzyme is essential for normal body growth and somatosensory development, as well as for the proper functioning of the adult mouse brain
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
show the reaction diagram
S-adenosyl-L-methionine + calmodulin L-lysine-115
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine-115
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + calmodulin L-lysine
S-adenosyl-L-homocysteine + calmodulin N6-methyl-L-lysine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ni2+
-
stimulation
additional information
-
not stimulating: Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmidazolium
p-Chloromercuriphenylsulfonic acid
-
-
S-adenosylhomocysteine
trimethyllysine calmodulin
-
-
-
VU-3 Calmodulin
-
VU-4 Calmodulin
-
site-specific mutant of calmodulin, Lys to Ile-115
-
additional information
-
not inhibitory: sinefungin, tubercidin, calmodulin antagonist W7
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
stimulation
Calmodulin
-
-
dithiothreitol
Triton X-100
-
-
[Thr136]calmodulin
-
defective in Ca-binding, cannot be methylated by the enzyme
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00001
Calmodulin
-
-
0.00023
mushroom demethylcalmodulin
-
-
-
0.00002
Octopus calmodulin
-
-
-
0.0008 - 0.002
S-adenosyl-L-methionine
0.00005
VU-1 calmodulin
-
-
-
additional information
additional information
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00031
trimethyllysine calmodulin
-
-
-
0.0004
VU-3 Calmodulin
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
-
additional information
-
pH 8.0, 7.8 or 6.0 depending on assay method
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
found at high levels in tissues with high levels of calmodulin, at low levels in tissues with low levels of calmodulin like liver, heart, skeletal muscle
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
gel filtration
55000
-
gel filtration
57000
-
gel filtration
65000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 37000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Triton stabilizes during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, up to 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana OX-1 and OX-7 strains in root tips, floral buds, stamens, apical meristems, and germinating seeds
-
expressed in COS-7 cells
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expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)-star-RIL cells
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