Information on EC 2.1.1.65 - licodione 2'-O-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.65
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RECOMMENDED NAME
GeneOntology No.
licodione 2'-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + licodione = S-adenosyl-L-homocysteine + 2'-O-methyllicodione
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
echinatin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:licodione 2'-O-methyltransferase
As well as licodione [1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione], the 2''-hydroxy-derivative and isoliquiritigenin can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
77000-07-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2'-deoxylicodione
S-adenosyl-L-homocysteine + ?
show the reaction diagram
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very poor substrate
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-
?
S-adenosyl-L-methionine + 2'-hydroxylicodione
S-adenosyl-L-homocysteine + ?
show the reaction diagram
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methylated at 38% of licodione methylation
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-
?
S-adenosyl-L-methionine + 2,4-dihydroxybenzoic acid
S-adenosyl-L-homocysteine + 2-methoxy-4-hydroxybenzoic acid
show the reaction diagram
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methylated at 4.5% of licodione methylation
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-
?
S-adenosyl-L-methionine + dihydroisoliquiritigenin
S-adenosyl-L-homocysteine + 2'-O-methyldihydroisoliquiritigenin
show the reaction diagram
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methylated at 7.2% of licodione methylation
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-
?
S-adenosyl-L-methionine + echinatin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
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methylated at 4% of licodione methylation
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-
?
S-adenosyl-L-methionine + isoliquiritigenin
S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
show the reaction diagram
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methylated at 10.7% of licodione methylation
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-
?
S-adenosyl-L-methionine + licodione
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
show the reaction diagram
S-adenosyl-L-methionine + resacetophenone
S-adenosyl-L-homocysteine + ?
show the reaction diagram
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methylated at 2.6% of licodione methylation
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + licodione
S-adenosyl-L-homocysteine + 2'-O-methyllicodione
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
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80% loss of activity
Zn2+
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30% loss of activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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slight increase of activity
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011
licodione
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0.0028
S-adenosyl-L-methionine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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cultured
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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