Information on EC 2.1.1.71 - phosphatidyl-N-methylethanolamine N-methyltransferase

Word Map on EC 2.1.1.71
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.71
-
RECOMMENDED NAME
GeneOntology No.
phosphatidyl-N-methylethanolamine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine
show the reaction diagram
the enzyme also catalyzes the transfer of a further methyl group, producing phosphatidylcholine
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
phosphatidylcholine biosynthesis III
-
-
phosphatidylcholine biosynthesis IV
-
-
phosphatidylcholine biosynthesis V
-
-
phosphatidylethanolamine bioynthesis
-
-
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:phosphatidyl-N-methylethanolamine N-methyltransferase
The enzyme also catalyses the transfer of a further methyl group, producing phosphatidylcholine.
CAS REGISTRY NUMBER
COMMENTARY hide
67167-73-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
mouse
-
-
Manually annotated by BRENDA team
rabbit
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
strain Wistar
-
-
Manually annotated by BRENDA team
fission yeast
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
PEMT-L and PEMT-S expression increase the cellular phosphatidylcholine content, whereas the phosphatidylethanolamine content is decreased by PEMT-S expression, but is hardly affected by PEMT-L expression. The cellular content of phosphatidylserine is also reduced by the expression of PEMT-L or PEMT-S
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + dipalmitoylphosphatidyl-N,N-dimethylethanolamine
S-adenosyl-L-homocysteine + dipalmitoylphosphatidylcholine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + dipalmitoylphosphatidyl-N-methylethanolamine
S-adenosyl-L-homocysteine + dipalmitoylphosphatidyl-N,N-dimethylethanolamine dipalmitoylphosphatidyl-N-methylethanolamine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N,N-dimethylethanolamine
S-adenosyl-L-homocysteine + phosphatidylcholine
show the reaction diagram
S-adenosyl-L-methionine + phosphatidyl-N-ethanolamine
S-adenosyl-L-homocysteine + phosphatidyl-N-monomethylethanolamine
show the reaction diagram
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine
S-adenosyl-L-homocysteine + phosphatidyl-N,N-dimethylethanolamine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine
show the reaction diagram
S-adenosyl-L-methionine + phosphatidyl-N-monomethylethanolamine
S-adenosyl-L-homocysteine + phosphatidylcholine
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
leads to a stimulation of activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adriamycin
-
doxorubicin, antitumor drug
octyl glucoside
-
-
p-Chloromercuriphenylsulfonic acid
-
-
phosphatidylcholine
-
inhibits the incorporation of [3H]-methyl groups from S-adenosyl-L-methionine into phosphatidyl-N-monomethylethanolamine and phosphatidyl-N,N-dimethylethanolamine
S-adenosyl-L-homocysteine
sodium cholate
-
-
Triton X-100
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.059 - 0.18
phosphatidyl-N-dimethylethanolamine
0.054 - 0.38
phosphatidyl-N-monomethylethanolamine
0.016 - 0.1
S-adenosyl-L-methionine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0049 - 0.32
S-adenosyl-L-homocysteine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity of PEMT-S is much higher than of its isoform PEMT-L
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
8.5
-
low substrate concentrations about 0.01 M S-adenosyl-L-methionine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
-
-
6.5 - 10.5
-
-
7 - 11
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
synaptosomes
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
skeletal muscle
Manually annotated by BRENDA team
-
islet cells
Manually annotated by BRENDA team
-
microvilli
Manually annotated by BRENDA team
-
rod outer segments
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19000
-
SDS-PAGE, PEMT-S
23150
-
predicted from amino acid sequence
26000
-
SDS-PAGE, PEMT-L
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
N-glycosylated with high-mannose oligosaccharides
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
unstable above
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, not stable to freezing
-
2°C, loses 30% of its activity within 12 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme is encoded by PEM2/OPI3 gene, cloned by complementation
-
expressed in Saccharomyces cerevisiae
stably expressed in human embryonic kidney-293 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression level of PEMT-L is 4.4fold higher than that of PEMT-S
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine