Information on EC 2.1.1.75 - apigenin 4'-O-methyltransferase

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The expected taxonomic range for this enzyme is: Robinia pseudoacacia

EC NUMBER
COMMENTARY hide
2.1.1.75
-
RECOMMENDED NAME
GeneOntology No.
apigenin 4'-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + apigenin = S-adenosyl-L-homocysteine + acacetin
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
para-O-methylation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acacetin biosynthesis
-
-
Flavone and flavonol biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:5,7,4'-trihydroxyflavone 4'-O-methyltransferase
Converts apigenin into acacetin. Naringenin can also act as an acceptor, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
118251-36-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
S-adenosyl-L-homocysteine + 4'-methoxy-5,7-dihydroxyflavone
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
S-adenosyl-L-homocysteine + 4'-methoxy-5,7-dihydroxyflavone
show the reaction diagram
-
enzyme is involved in biosynthesis of 4'-methoxylated flavonoids
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-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
-
0.1 mM, 47% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
5 mM, required for optimum activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
apigenin
-
-
0.031
S-adenosyl-L-methionine
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
-
glycine-NaOH buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10
-
about 50% of activity maximum at pH 7.5 and 10, glycine-NaOH buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE