Information on EC 2.1.1.85 - protein-histidine N-methyltransferase

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The expected taxonomic range for this enzyme is: Oryctolagus cuniculus

EC NUMBER
COMMENTARY hide
2.1.1.85
-
RECOMMENDED NAME
GeneOntology No.
protein-histidine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-histidine N-tele-methyltransferase
Highly specific for histidine residues, for example, in actin.
CAS REGISTRY NUMBER
COMMENTARY hide
108022-17-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
show the reaction diagram
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for any metal ion
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
47% inhibition at 0.4 mM
Borate
-
slight inhibition
Ca2+
-
30% inhibition at 0.4 mM
EDTA
-
slight inhibition
K+
-
72% inhibition at 200 mM
Mg2+
-
60% inhibition at 8 mM
N-ethylmaleimide
-
70% inhibition at 4 mM
Na+
-
95% inhibition at 200 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
stimulation at 1%
additional information
-
reducing agents e.g. mercaptoethanol, required for activity, 4fold stimulation
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
different fractions after ammonium sulfate precipitation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 9
-
about 50% of activity maximum at pH 6.8 and 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
10 min, 75% loss of activity
50
-
10 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol or glycerol does not stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2°C, 4 days, 75% loss of activity, no stabilization by mercaptoethanol or glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE