Information on EC 2.1.1.94 - tabersonine 16-O-methyltransferase

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The expected taxonomic range for this enzyme is: Catharanthus roseus

EC NUMBER
COMMENTARY hide
2.1.1.94
-
RECOMMENDED NAME
GeneOntology No.
tabersonine 16-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 16-hydroxytabersonine = S-adenosyl-L-homocysteine + 16-methoxytabersonine
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
vindoline and vinblastine biosynthesis
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-
Indole alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:16-hydroxytabersonine 16-O-methyltransferase
Involved in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle, Catharanthus roseus.
CAS REGISTRY NUMBER
COMMENTARY hide
100984-95-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 11-O-demethyl-17-O-deacetylvindoline
S-adenosyl-L-homocysteine + 17-O-deacetylvindoline
show the reaction diagram
S-adenosyl-L-methionine + 16-hydroxytabersonine
S-adenosyl-L-homocysteine + 16-methoxytabersonine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 11-O-demethyl-17-O-deacetylvindoline
S-adenosyl-L-homocysteine + 17-O-deacetylvindoline
show the reaction diagram
-
involved in biosynthesis of the alkaloid vindoline
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-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
-
product inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
16-hydroxytabersonine
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-
0.0217
S-adenosyl-L-methionine
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
S-adenosyl-L-homocysteine
-
product inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000417
-
16-hydroxytabersonine as a substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
specifically located in the epidermis of aerial organs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using PD-10 column chromatography, Sephadex G150 column chromatography, adenosine-agarose affinity resin and Mono Q anion exchange column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
16-hydroxytabersonine-16-O-methyltransferase is isolated and cloned as a His-tagged fusion protein in Escherichia coli
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