Information on EC 2.1.1.B85 - 7'-O-demethylcephaline methyltransferase

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The expected taxonomic range for this enzyme is: Carapichea ipecacuanha

EC NUMBER
COMMENTARY hide
2.1.1.B85
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
7'-O-demethylcephaline methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:7'-O-demethylcephaeline 7'-O-methyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
CiOMT2 catalyzes the penultimate step, 7-O'-methylation, in emetine biosynthesis, the recombinant CiOMT2 also the last step, synthesis of emetine by 6'-O-methylation, pathway overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 7'-O-demethylcephaeline
S-adenosyl-L-homocysteine + cephaeline
show the reaction diagram
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
show the reaction diagram
the enzyme is involved in emetine biosynthesis. IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 7'-O-demethylcephaeline
S-adenosyl-L-homocysteine + cephaeline
show the reaction diagram
E7FL15
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-
-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
show the reaction diagram
D3KY99
the enzyme is involved in emetine biosynthesis. IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline
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-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
no effect by EDTA, Ca2+, and Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
53% inhibition at 5 mM
Mn2+
31% inhibition at 5 mM
Ni2+
83% inhibition at 5 mM
Zn2+
90% inhibition at 5 mM
additional information
poor inhibition by iodoacetamide
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38700, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-terminal His-tagged IpeOMT2 enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
OMT2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)