Information on EC 2.1.1.B93 - N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces davawensis

EC NUMBER
COMMENTARY hide
2.1.1.B93
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
8-amino-8-demethyl-D-riboflavin + 2 S-adenosyl-L-methionine = roseoflavin + 2 S-adenosyl-L-homocysteine
show the reaction diagram
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine = 8-methylamino-8-demethyl-D-riboflavin + S-adenosyl-L-homocysteine
show the reaction diagram
8-methylamino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine = roseoflavin + S-adenosyl-L-homocysteine
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:8-amino-8-demethyl-D-riboflavin N8-methyltransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0577
8-amino-8-demethyl-D-riboflavin
pH 8.0, 37C
0.0286
S-adenosyl-L-methionine
pH 8.0, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.37
8-amino-8-demethyl-D-riboflavin
Streptomyces davawensis
E7BBN5
pH 8.0, 37C
0.24
S-adenosyl-L-methionine
Streptomyces davawensis
E7BBN5
pH 8.0, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl-Sepharose chromatography, anion exchange chromatography, gel filtration on a HiLoad 16/60 Superdex 200 column, analyzed using SDS-PAGE and MS/MS
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta 2 (DE3), recombinant His6-tagged RosA purified, ORF sda77220 is named rosA is located in the middle of a putative gene cluster within the genome of Streptomyces davawensis