Information on EC 2.3.1.100 - [myelin-proteolipid] O-palmitoyltransferase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
2.3.1.100
-
RECOMMENDED NAME
GeneOntology No.
[myelin-proteolipid] O-palmitoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
palmitoyl-CoA + [myelin proteolipid] = CoA + O-palmitoyl-[myelin proteolipid]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
palmitoyl-CoA:[myelin-proteolipid] O-palmitoyltransferase
The enzyme in brain transfers long-chain acyl residues to the endogenous myelin proteolipid
CAS REGISTRY NUMBER
COMMENTARY hide
82657-98-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
myristoyl-CoA + myelin proteolipid protein
CoA + myelin proteolipid O-myristoylprotein
show the reaction diagram
-
low activity
-
-
?
oleoyl-CoA + myelin proteolipid protein
CoA + myelin proteolipid O-oleoylprotein
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + myelin proteolipid protein
CoA + myelin proteolipid O-palmitoylprotein
show the reaction diagram
-
-
-
?
stearoyl-CoA + myelin proteolipid protein
CoA + myelin proteolipid O-stearoylprotein
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme is probably involved in the acylation of endogenous myelin proteolipid protein
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
palmitoyl-CoA + myelin proteolipid protein
CoA + myelin proteolipid O-palmitoylprotein
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme is probably involved in the acylation of endogenous myelin proteolipid protein
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
-
2 mM, stimulates
MnCl2
-
stimulates
NaF
-
slight stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sodium dodecyl sulfate
-
at concentration 0.5%: 92% loss of activity
Tween 20
-
inhibits slightly at 0.5%
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
1 mM, slightly stimulates
Nonidet P-40
-
stimulates at 0.05%
octyl glucoside
-
slight stimulation
Sodium deoxycholate
-
stimulates at 0.5%
Triton X-100
-
0.1%, stimulates
Tween 20
-
0.05%, slight stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.219
myristoyl-CoA
-
-
0.046
oleoyl-CoA
-
-
0.041
palmitoyl-CoA
-
-
0.05
stearoyl-CoA
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
25% of maximal activity at pH 6, 90% of maximal activity at pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
-
45% of maximal acticity at 10°C, 75% of maximal activity at 50°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
labeled product also found in vitro in brain tissue slices
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
-
5 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing causes marked decrease in activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE